| 健康家兔服用大川芎丸后体内阿魏酸的药代动力学研究 |
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| 引用本文： | 王兴. 健康家兔服用大川芎丸后体内阿魏酸的药代动力学研究[J]. 成都医学院学报, 2008, 3(1): 1-4 |
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| 作者姓名： | 王兴 |
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| 作者单位： | 西南交通大学药学院,四川省峨眉山市,614202;现代中药制剂教育部重点实验室,南昌市,330004 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的建立健康家兔服用大川芎丸后血清中阿魏酸（FA）的高效液相色谱测定法（HPLC）,并研究阿魏酸在大鼠体内的药代动力学。方法用HPLC法,色谱柱为Inertsil ODS-3（5μm,4.6×250mm）,流动相为：甲醇∶水∶冰乙酸（49∶50.745∶0.255,V/V）,流速1.0mL·min^-1,检测波长322nm。FA用内标（香豆精）法定量。结果FA的最低检测限为0.25ng,最低血清检测浓度为5ng·mL^-1（S/N〉3）,线性范围是26.4-4224ng·mL^-1,r=0.9999.平均加样回收率为98.17%±1.26%.结论该方法灵敏、快速、简便、专一性和重现性好,可用于阿魏酸血药浓度分析及药代动力学研究。
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| 关 键 词： | 大川芎丸 阿魏酸 血清 家兔 HPLC |
Pharmacokinetics of Ferulic Add in Rabbit after Administering DACHUANXIONGWAN |
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| WANG Xing. Pharmacokinetics of Ferulic Add in Rabbit after Administering DACHUANXIONGWAN[J]. Journal of Chengdu Medical College, 2008, 3(1): 1-4 |
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| Authors: | WANG Xing |
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| Affiliation: | WANG Xing(1.School of Pharmacy,Southwest Jiaotong University,Emeishan City,Sichuan Province 61420,China;2.Key Laboratory of Modern TCM Preparation,Ministry of Education,Nanchang City,Jiangxi Province 33000,China) |
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| Abstract: | objective To establish the method for determining ferulic acid in serum by HPLC and study the pharmacokinetics in healthy rabbit after taken DCXW.Methods Inertsil ODS-3（5 μm,4.6×250 mm）was used as the stationary phase,methanol-Water-acetic acid（49∶50.745∶0.255,V/V）was used as mobile phase,flow rate of mobile phase was 1.0 mL·min^-1,wavelength of detection was 322 nm.The Coumarin was used as inner standard.Results The detection limit of FA was 0.25 ng,and the lowest detectable concentration of FA was 5 ng·mL^-1,with a linearity of 26.4-4224 ng·mL^-1,r=0.9999.The mean recovery rate of FA was 98.17%±1.26%.Conclusion This method is sensitive,fast,simple,specific and reproducible.It can be used to the analysis of FA in serum and the study of pharmacokinetics. |
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| Keywords: | HPLC |
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