固相萃取-超高效液相色谱荧光法测定植物油中苯并(a)芘

采用中性氧化铝小柱净化样品，建立固相萃取-超高效液相色谱荧光法测定植物油中苯并(a)芘的方法。样品以正己烷为提取剂，净化、蒸发浓缩后正己烷溶解，荧光检测器检测。流动相为乙腈-水(75:25，V/V)，流速0.5mL/min，外标法定量。苯并(a)芘在0.10～50.0μg/L质量浓度范围内线性关系良好，相关系数R2为0.9998；在空白样品中添加3个水平的标准品，回收率在91.7%～97.5%之间，相对标准偏差均小于2%(n＝3)；最低检出限为0.01μg/kg，定量限为0.03μg/kg。方法操作简便、分析时间短、稳定性好、选择性好、灵敏度高，为植物油中苯并(a)芘的测定提供参考。

Determination of Benzopyrene in Cooking Oil by Solid-Phase Extraction Coupled with Ultra Performance Liquid Chromatograph-Fluorescence Detection

A method was developed for the determination of benzopyrene by solid-phase extraction on a neutral Al2O3 cartridge coupled with ultra performance liquid chromatography (UPLC). Benzopyrene in samples were extracted into n-hexane, cleaned up, and concentrated by rotary evaporation and dissolved in n-hexane again before chromatographic separation and fluorescence detection. The mobile phase was acetonitrile-water (75:25, V/V) at a flow rate of 0.5 mL/min. Benzopyrene was quantified by external standard method. A good linear relationship between peak area and benzopyrene concentration was achieved in the range of 0.10－50.0 μg/L (R2 ＝ 0.9998). The mean spike recovery rates of benzopyrene at three concentration levels were in the range of 91.7%－97.5%, with relative standard deviation (RSD) of less than 2%. The limits of detection (LOD) and quantification (LOQ) were 0.01 μg/kg and 0.03 μg/kg, respectively. The developed method is characteristics of simple operation, good reproducibility, high sensitivity, and rapid, reliable and suitable determination for benzopyrene in cooking oil.