纳米SiO2对大鼠肺泡Ⅰ型上皮细胞毒性的影响

目的 探讨纳米SiO2的肺细胞毒性.方法 用透射电镜观察2种不同粒径纳米SiO2粒子粒径、分散性、形状;用Zeta电位粒度分析仪检测纳米粒子在纯水和含体积分数为1%胎牛血清的RPMI 1640细胞培养液中的粒径.以大鼠肺泡Ⅰ型上皮细胞R3/1为靶细胞,利用分光光度法检测浓度均为2.5、5.0、10.0、20.0 μg/ml的2种不同粒径纳米SiO2粒子在6、24和48 h引起细胞乳酸脱氢酶(LDH)活力的变化;用酶联免疫法检测24 h时2种纳米SiO2粒子对细胞蛋白质羰基化水平及巨噬细胞炎性蛋白2(MIP-2)释放的影响.结果 2种粒径纳米SiO2粒子均呈球形,大小一致,分布均匀,分散性好,平均粒子粒径分别为(43±4.2)和(68±5.7)nm;2种粒子分别在纯水和含质量分数为1%胎牛血清的RPMI 1640细胞培养液中有相似的粒径.6、24、48 h,2.5、5.0、10.0和20.0μg/ml的43和68 nm粒子引起细胞LDH活力轻微增加,与对照组相比,差异无统计学意义(P＞0.05);作用24 h后,细胞MIP-2的释放及细胞羰基化水平轻度增加,与对照组相比,差异均无统计学意义(P＞0.05).结论 在该实验条件下,2.5、5.0、10.0、20.0 μg/ml的2种纳米粒子对R3/1细胞无明显肺毒性.

Toxicity of nano silica particles on rat type I-like alveolar epithelial cells

objective To access rat lung toxicity of nano silica particles.Methods Transmission electron microscope was used to observe size,shape and dispersibility of two silica particles;Size of two Dartieles in water and RPMI 1640 containing 1%FBS were measured using Zeta Potential Analyzer.LDH activities of rat type I-like alveolar epithelial cell line R3/1 cells after 6,24 and 48 h incubation with 2.5,5.0,1 0.0,20.0 μg/ml of two silica nano particles were detected by spectmphotometric methud;protein carbonylation and MIP-2 release of R3/1 cells after 24 h incubation with 2.5,5.0,10.0,20.0μg/ml of two silica nano particles were measured using ELISA kits.Results TEM image showed nano silica particles were round and dispersed evenly;the average sizes of the two silica particles were(43±4.2)and(68±5.7)nm.Two silica particles had similar size in water and RPMI 1640 containing 1%FBS.respectively.Both nano silica particles in 2.5～20.0μg/nd dose range did not cause significant increase of LDH activities(P＞0.05),did not elevate protein carbonylation and MIP-2 levels in R3/1 cells(P＞0.05).Conclusion Two nano silica particles do not have lung toxicity in 2.5～20.0 p.g/ml dose range.