烟草PVY隐性抗病基因的分子标记及其适用性

马铃薯Y 病毒(Potato virus Y,PVY)是危害烟草的重要病害, 种植抗病品种是经济有效的防治措施。分子标记辅助选择可提高抗病育种效率。来源于X-射线诱变的Virgin A Mutante(VAM) 的隐性抗PVY 基因位点(va)被广泛应用于烟草抗病育种。为了提高va 位点的育种利用效率, 根据烟草隐性抗PVY 基因(感病基因)eIF4E-1 基因序列, 设计特异扩增的引物CF2GR11,开发eIF4E-1 基因的分子标记, 并检测了该标记与抗性的遗传距离和在常见烟草资源中的适用性。CF2GR11 在云烟87、红花大金元和K326 等感PVY 品种可扩增出500 bp 产物, 在NC102、NC55 和K326PVY 等抗病品种无扩增条带。以抗、感PVY 亲本构建的101 个F₂ 单株为定位群体, 遗传连锁分析表明, CF2GR11 标记与烟草PVY 感病基因的遗传距离为0.99 cM。对46 份PVY 抗性明确的栽培烟草资源的检测表明, 供试资源的标记检测结果与抗性的吻合度为100%, 表明CF2GR11 标记适用性高。该目的基因标记可用于抗PVY 育种的辅助选择和抗PVY 资源的鉴定。 

Molecular marker from recessive gene resistant to potato virus Y of tobacco and its suitability

Potato virus Y (PVY) is a serious disease of tobacco. To plant resistant varieties is an economical and effective control measure. Molecular marker-assisted selection could improve breeding efficiency. A gene locus va of Virgin A Mutante (VAM) induced by X-ray irradiation is widely used in tobacco breeding. In order to improve va locus breeding utilization efficiency, specific amplification primer CF2GR11 was developed based on recessive resistance gene eIF4E-1 (susceptible gene) to PVY of tobacco, and genetic distance between CF2GR11 and resistance gene, as well as its suitability of CF2GR11 in tobacco germplasm were evaluated. In PVY susceptible varieties Yunyan87, Honghuadajinyuan and K326, CF2GR11 can produce a 446bp product as amplifier, while in PVY resistant varieties NC102, NC55 and K326PVY no product came out. Genetic linkage analysis with F2 population from a PVY resistant and a susceptible parent as locus point showed that genetic distance between CF2GR11 marker and resistance gene was 0.99cM. CF2GR11 proved suitable in 46 tested tobacco germplasm. This target gene marker could be used as an assisted selection tool for PVY resistant cultivars breeding and germplasm identification.