| 检测抗丙型肝炎病毒总抗体的双抗原夹心法的建立 |
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| 引用本文： | 洪世雯 貌盼勇. 检测抗丙型肝炎病毒总抗体的双抗原夹心法的建立[J]. 中华实验和临床病毒学杂志, 1997, 11(4): 375-377 |
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| 作者姓名： | 洪世雯 貌盼勇 |
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| 作者单位： | 解放军３０２医院 |
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| 摘 要： | 以丙型肝炎病毒（ＨＣＶ）结构区和非结构区基因工程表达抗原及人工合成多肽包被酶标板，用辣根过氧化物酶标记抗原，建立了检测血清中抗－ＨＣＶ总抗体的双抗原夹心法。与普遍采用的检测抗－ＨＣＶＩｇＧ的间接酶联免疫吸附试验（ＥＬＩＳＡ）比较，其灵敏度（１∶１２８）稍高于间接法（１∶６４），特异性相同，均为１００％。该法可同时检测抗－ＨＣＶ各类抗体，使检出率提高１０％。
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| 关 键 词： | 肝炎病毒.丙型 抗原.病毒 抗体.病毒 酶联免疫吸附测定 |
The establishment of antigen sandwiched ELISA for detection of total antibodies to hepatitis C virus |
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| S Hong,P Mao,H He,T Fu,H Liu,Y Bai,J Yang. The establishment of antigen sandwiched ELISA for detection of total antibodies to hepatitis C virus[J]. Chinese journal of experimental and clinical virology, 1997, 11(4): 375-377 |
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| Authors: | S Hong P Mao H He T Fu H Liu Y Bai J Yang |
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| Affiliation: | Department of Virology, 302 Hospital, Beijing 100039. |
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| Abstract: | An antigen sandwiched ELISA for detection of the total antibodies to HCV was established using genetically engineered proteins and synthesized peptides located in the structural and nonstructural regions of HCV genomes as coated antigens and were labeled by horseradish peroxidase, respectively. A comparison of antigen sandwiched ELISA with indirect ELISA showed that the sensitivity of sandwiched system (1:128) was little higher than that of indirect ELISA (1:64), the specificities of both systems were all 100%. This reagent may detect all classes of antibody to HCV in serum, mainly IgG and IgM and is a new diagnostic reagent for detection and early diagnosis of HCV infection. |
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