血清乙型肝炎病毒前S1抗原检测及其与病毒复制的关系

用抗Ｓ和抗前Ｓ１单抗的双抗体夹心ＥＬＩＳＡ法检测１５０例慢性乙型肝炎患者、乙型肝炎病毒表面抗原（ＨＢｓＡｇ）携带者和健康人血清中的ＨＢＶ前Ｓ１抗原，其结果和ＨＢＶＤＮＡ聚合酶链反应（ＰＣＲ）、乙型肝炎血清标志的检测结果进行比较。结果表明：前Ｓ１抗原在乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）阳性组中的检出率和相对滴度显著高于ＨＢｅＡｇ阴性组（Ｐ＜０．０１）；在ＨＢｅＡｇ阴性组中，抗－ＨＢｅ阴性人群前Ｓ１抗原的检出率和相对滴度也显著高于抗－ＨＢｅ阳性人群（Ｐ＜０．０１）。前Ｓ１抗原和ＨＢＶＤＮＡ检测结果的符合率达８０％，两者检出率的相关系数ｒ＝０．９８２６（Ｐ＜０．０１）。结论：血清前Ｓ１抗原和乙型肝炎病毒的存在关系密切。

The detection of serum hepatitis B virus Pre-S1 antigen and its relationship with HBeAg/ anti-HBe and HBV DNA by polymerase chain reaction

150 specimens were collected from chronic hepatitis patients, asymptomatic hepatitis B surface antigen (HBsAg) carriers and healthy individuals. Serum Pre-S1 and HBV markers were determined by enzyme immunoassay (EIA) and HBV DNA was tested by polymerase chain reaction (PCR). According to the results, specimens could be classified into five different groups: HBsAg and hepatitis e antigen (HBeAg) both positive; HBsAg positive and HBeAg/anti-HBe both negative; HBsAg and anti-HBe both positive with HBeAg negative; HBsAg negative with positive antibodies to s, c and e antigens; negative in all HBV markers. Each groups comprised 30 specimens respectively. The positive rate and relative titer (sample A value/cut-off value) of Pre-S1 Ag in HBeAg positive group (66.6%, 4.3250 +/- 2.2013, respectively) were markedly higher than those in HBeAg negative group (31.6%, 1.7863 +/- 0.6339, respectively, P<0.01). The detection rate of Pre-S1 Ag in these samples were 82% coincided with that of HBV DNA. The positive rate of Pre-S1 in the sera from five groups with different HB markers were well correlated to that of HBV DNA (r=0.9826, P<0.01). These results suggest that serum Pre-S1 detection can be used as a marker for the presence of the HBV genome and its quantitation will be correlated with the replication of virus.