不同贝壳煅烧粉的杀菌及抑菌效果

目的:研究不同贝壳煅烧粉对致病菌的杀菌及抑菌效果。方法:将贻贝、文蛤、溢蛏3种贝壳高温煅烧1000℃,2 h,并配制成1%、0.1%、0.01%浓度的煅烧粉悬浊液,作用于沙门氏菌、副溶血弧菌、金黄色葡萄球菌以及大肠杆菌,利用菌液杀菌以及培养基扩散抑菌方法,检测3种贝壳煅烧粉对不同致病菌的杀菌及抑菌效果。结果:3种贝壳煅烧粉对4种菌均具有杀菌作用,对于革兰氏阴性菌的杀菌作用明显大于革兰氏阳性菌,作用最强为缢蛏及文蛤贝壳煅烧粉对副溶血弧菌,分别作用5 min后菌落总数小于10 CFU/mL。培养基扩散法的抑菌实验表明,3种贝壳煅烧粉对4种菌均有抑菌作用,抑菌效果最强为缢蛏贝壳煅烧粉在0.1 g/mL浓度下作用于沙门氏菌,抑菌圈直径达16.42 mm;在各浓度阶段,贻贝和文蛤贝壳煅烧粉对副溶血弧菌的抑菌性明显强于于沙门氏菌以及大肠杆菌。结论:贝壳煅烧粉对食源性致病菌杀菌及抑菌作用明显,可用作抑菌剂。

Bactericidal and Inhibitory Effect of Different Calcined Shell Powder

Objective:to study the bactericidal and inhibitory effect of different calcined shell powder on pathogenic bacteria. Methods:Shells of Mussels,Meretrix meretrix and Sinonovacula constricta were calcined at a high temperature of 1000℃ for 2 h,and diluted into 1%,0.1%,0.01% concentration of calcination powder suspension. The bactericidal and inhibitory effect of 3 kinds of calcined shell powder on Salmonella,Vibrio parahaemolyticus,Staphylococcus aureus and Escherichia coli were detected using bacteria sterilization and culture medium diffusion method. Results:The calcined shell powder had bactericidal effects on 4 kinds of bacteria,and gram negative bacteria better than gram positive bacteria. Sinonovacula constricta and Meretrix meretrx calcined shell powder on Vibrio parahaemolyticus was the strongest,the number of bacteria was less than 10 CFU/mL after 5 min.The inhibitory experiment of the medium diffusion method showed that 3 kinds of calcined shell powder had inhibitory effect on 4 kinds of bacteria. Under the concentration of 0.1 g/mL,the inhibitory effect of Sinonovacula constricta calcined shell powder on Salmonella was the strongest and the diameter of inhibition zone was 16.42 mm. At each concentration,the inhibition of Mussels and Meretrix meretrix shell calcination powder on Vibrio parahaemolyticus was significantly stronger than that of Salmonella and Escherichia coli. Conclusion:The calcined shell powder as a inhibitor had obvious bactericidal and inhibitory effect on food borne pathogens.