白藜芦醇对晚期糖基化终末产物诱导INS-1细胞损伤的保护作用及机制

目的:研究白藜芦醇(RSV)对晚期糖基化终末产物(AGEs)诱导大鼠胰岛素瘤细胞(INS-1)损伤的保护作用及其抗炎抗氧化应激机制。方法:本实验使用含AGEs的糖化血清(GS)建立INS-1细胞损伤模型。实验分对照组、20% GS模型组、20% GS+不同浓度RSV组。检测各组细胞增殖率、细胞内超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)含量、培养液上清一氧化氮(NO)和细胞内活性氧(ROS)、丙二醛(MDA)含量以及培养液上清肿瘤坏死因子(TNF-α)含量。结果:与对照组比较,模型组INS-1细胞增殖率、SOD活性和GSH的含量极显著降低(p<0.01),NO和ROS含量极显著升高(p<0.01),MDA含量显著升高(p<0.05),TNF-α含量极显著降低(p<0.01)。与模型组比较,25和50 μmol/L的RSV组INS-1细胞增殖率均极显著提高(p<0.01),同时SOD活力和GSH含量极显著升高(p<0.01),NO的水平极显著降低(p<0.01)。此外,50 μmol/L的RSV组ROS含量显著降低(p<0.05),25 μmol/L的RSV组MDA含量显著降低(p<0.05),25和50 μmol/L的RSV组的TNF-α水平极显著降低(p<0.01)。结论:RSV对AGEs损伤的INS-1细胞有显著的保护作用,其作用机制可能是通过提高INS-1细胞的抗氧化应激能力和抗炎症反应能力实现的。

Protective Effect and Mechanism of Resveratrol on INS-1 Cell Injury Induced by Advanced Glycation End Products

Objective:The aim of this study was to investigate the protective effect of resveratrol(RSV)on the rat INS-1 cell injury induced by advanced glycation end products(AGEs)and the underlying mechanisms of anti-inflammatory and anti-oxidative stress involved in it. Methods:In this experiment,the glycation serum(GS)which included various AGEs was used to mimic the diabetes settings and to establish a cell injured model. INS-1 cells were divided into control group,20% GS model group,20% GS+RSV groups with different concentrations. The cell proliferation rates was detected by MTT assay. The intracellular SOD activities,GSH contents,supernatant NO,intracellular ROS,MDA contents and supernatant TNF-α were measured by Kits. Results:Compared with control group,the model group cells proliferation rate were significantly decreased(p<0.01).The SOD activities and the levels of GSH in model group were significantly decreased(p<0.01). The levels of NO and ROS in model group were significantly increased(p<0.01). The levels of MDA in model group were significantly increased(p<0.05).The levels of TNF-α in model group were significantly increased(p<0.01). Compared with model group,the proliferation rates of INS-1 cells of 25 and 50 μmol/L RSV group were significantly increased(p<0.01),and the content of GSH and SOD activities were significantly increased(p<0.01),the contents of NO were significantly decreased(p<0.01). In addition,the levels of MDA were significantly reduced(p<0.05)in 25 μmol/L RSV group and the levels of ROS were significantly reduced(p<0.05)in 50 μmol/L RSV group. The contents of TNF-α were significantly decreased(p<0.01). Conclusion:RSV has a significant protective effect on the AGEs injured INS-1 cells,and the mechanism would be related to improve the anti-oxidative stress and anti-inflammatory abilities of INS-1 cells.