血管紧张素II对增生性瘢痕成纤维细胞生长增殖的影响

目的:探讨血管紧张素II及其I型受体(AT1)拮抗剂洛沙坦(losartan)对增生性瘢痕成纤维细胞生长增殖的影响。方法:体外分离培养增生性瘢痕成纤维细胞,在不同浓度的血管紧张素II、losartan、PD123319作用下,观察成纤维细胞的生长情况,并绘制生长曲线,采用MTT法和3H-TDR掺入释放法分别检测成纤维细胞增殖和DNA含量的情况。结果:血管紧张素II在浓度为10-8mol/L～10-5mol/L时,成纤维细胞的细胞数量明显增多,DNA含量增加(P<0.05);losartan能几乎完全抑制AngII的这种作用,PD123319对此作用几乎没有影响。结论:AngII可促增生性瘢痕成纤维细胞的增殖,该作用主要通过AngII的I型受体介导完成,AT1的拮抗剂有望在增生性瘢痕防治中发挥重要作用。

The effects of angiotensin II on the growth and proliferation of hypertrophic scars-derived fibroblasts

Objective To investigate the effects of angiotensin II and AT1 blocker losartan on growth and proliferation of hypertrophic scars-derived fibroblasts.Methods Fibroblasts were freshly isolated from hypertrophic scars and cultured,followed by incubation with angiotensin II? losartan and PD123319 at different concentrations.The cell growth and proliferation were assessed via cell counting and MTT assay,and the effects of the agents on DNA synthesis of hypertrophic scars-derived fibroblasts were evaluated by way of 3H-thymidine incorporation(3H-TDR).Results Angiotensin II(10-8mol/L~10-5mol/L) stimulated hypertrophic scars-derived fibroblasts proliferation as demonstrated by cell counting,MTT assay and 3H-thymidine incorporation test(P<0.05).But this effect could be blocked completely by losartan,while PD123319 has no effect.Conclusion Angiotensin II promotes growth and proliferation of hypertrophic scars-derived fibroblasts obviously via AT1R.Losartan may play an important role in preventing and treating hypertrophic scars.