| 实时荧光RT-PCR检测冷冻草莓中诺如病毒 |
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| 引用本文： | 周阳,栾军,蒋鲁岩,唐泰山,张常印,张弛,杨军,付瑞燕,祝长青.实时荧光RT-PCR检测冷冻草莓中诺如病毒[J].食品安全质量检测技术,2013,4(2):515-520. |
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| 作者姓名： | 周阳 栾军 蒋鲁岩 唐泰山 张常印 张弛 杨军 付瑞燕 祝长青 |
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| 作者单位： | 安徽农业大学茶与食品科技学院，安徽省食品安全分析与检测实验室,江苏出入境检验检疫局,江苏出入境检验检疫局,江苏出入境检验检疫局,江苏出入境检验检疫局,江苏出入境检验检疫局,安徽农业大学茶与食品科技学院，安徽省食品安全分析与检测实验室,安徽农业大学茶与食品科技学院，安徽省食品安全分析与检测实验室,安徽农业大学茶与食品科技学院，安徽省食品安全分析与检测实验室 |
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| 基金项目： | 青奥会食品安全保障关键技术应用与示范项目(2011BAK21B05) |
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| 摘 要： | 目的：建立冷冻草莓中的GI、GII型诺如病毒实时荧光RT-PCR检测方法,并应用于实际样品的检测。方法：对草莓样品进行前处理、病毒富集、病毒RNA的提取和纯化,然后采用实时荧光RT-PCR进行检测。结果：核酸提取方法能够有效地去除抑制因子,同时对104份送检样品进行检测,结果均为阴性。结论：所建立的核酸提取与实时荧光RT-PCR结合的检测体系适合于草莓样品中诺如病毒GI、GII型的检测。
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| 关 键 词： | 冷冻草莓 诺如病毒 实时荧光RT-PCR 快速检测 |
| 收稿时间： | 2013/1/15 0:00:00 |
| 修稿时间： | 3/29/2013 6:44:34 PM |
Detection of norovirus in frozen strawberry by real-time fluorescent RT-PCR |
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| ZHOU Yang,LUAN Jun,JIANG Ru-Yan,TANG Tai-Shan,ZHANG Chang-Yin,ZHANG Chi,YANG Jun,FU Rui-Yan and ZHU Chang-Qing.Detection of norovirus in frozen strawberry by real-time fluorescent RT-PCR[J].Food Safety and Quality Detection Technology,2013,4(2):515-520. |
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| Authors: | ZHOU Yang LUAN Jun JIANG Ru-Yan TANG Tai-Shan ZHANG Chang-Yin ZHANG Chi YANG Jun FU Rui-Yan and ZHU Chang-Qing |
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| Affiliation: | Anhui Provincial Key Laboratory of Analysis and Detection Technology for Food safety, School of Tea and Food Science , Anhui Agricultural University,Jiangsu Entry-exit Inspection and Quarantine Bureau,Jiangsu Entry-exit Inspection and Quarantine Bureau,Jiangsu Entry-exit Inspection and Quarantine Bureau,Jiangsu Entry-exit Inspection and Quarantine Bureau,Jiangsu Entry-exit Inspection and Quarantine Bureau,Anhui Provincial Key Laboratory of Analysis and Detection Technology for Food safety, School of Tea and Food Science , Anhui Agricultural University,Anhui Provincial Key Laboratory of Analysis and Detection Technology for Food safety, School of Tea and Food Science , Anhui Agricultural University,Anhui Provincial Key Laboratory of Analysis and Detection Technology for Food safety, School of Tea and Food Science , Anhui Agricultural University |
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| Abstract: | objective: to establish the real-time fluorescence RT - PCR detection method of norovirus GI and GII in frozen strawberry, and apply to actual sample testing. Methods: the strawberry sample pretreatment, concentration of viruses, virus RNA and purification, and then by using real-time fluorescence RT - PCR testing. Results: the nucleic acid extraction method can effectively remove the inhibitory factors. According to the test of one hundred inspection samples, the results are all negative. Conclusion: the established system of nucleic acid extraction and real-time fluorescence RT - PCR detection method is suitable for the detection of norovirus GI and GII in frozen strawberry samples. |
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| Keywords: | norovirus frozen strawberry real-time fluorescence RT-PCR rapid detection |
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