| B16F10/ESAT-6-GPI-IL-21瘤苗安全性及抗肿瘤效应研究 |
| |
| 引用本文： | 何向锋,王净,余方流,赵枫姝,蔡凯,张洪义,陈峻崧,陈登宇,窦骏.B16F10/ESAT-6-GPI-IL-21瘤苗安全性及抗肿瘤效应研究[J].中国免疫学杂志,2011,27(9):779-782. |
| |
| 作者姓名： | 何向锋 王净 余方流 赵枫姝 蔡凯 张洪义 陈峻崧 陈登宇 窦骏 |
| |
| 作者单位： | 1. 东南大学医学院病原生物学与免疫学系,南京,210009
2. 东南大学附属中大医院妇产科,南京,210009 |
| |
| 摘 要： | 目的:评估B16F10/ESAT-6-GPI-IL-21瘤苗用于C57BL/6小鼠的安全性及其诱导的抗肿瘤免疫效应。方法:应用免疫荧光、FCM检测瘤苗细胞ESAT-6-GPI的表达情况;以Western blot方法检测瘤苗细胞IL-21的表达情况;动物实验检测瘤苗体内应用的安全性;制备荷瘤鼠术后免疫模型,评价瘤苗免疫效果。结果:B16F10/ESAT-6-GPI-IL-21瘤苗细胞表面有靶抗原ESAT-6-GPI表达,并分泌IL-21。于C57BL/6小鼠皮下接种2×105个B16F10/ESAT-6-GPI-IL-21瘤苗细胞,60天内未见致瘤,但能够诱导小鼠产生有效的抗肿瘤免疫效应。结论:B16F10/ESAT-6-GPI-IL-21瘤苗致瘤性明显下降,低剂量应用具有安全性,能够诱导小鼠产生有效的抗肿瘤免疫效应。
|
| 关 键 词： | B16F10细胞 IL-21 ESAT-6 GPI 肿瘤疫苗 |
Study of safety and anti-tumor efficacy of B16F10/ESAT-6-GPI-IL-21 tumour vaccine in mouse model |
| |
| HE Xiang-Feng,WANG Jing,YU Fang-Liu,ZHAO Feng-Shu,CAI Kai,ZHANG Hong-Yi,CHEN Jun-Song,CHEN Deng-Yu,DOU Jun.Study of safety and anti-tumor efficacy of B16F10/ESAT-6-GPI-IL-21 tumour vaccine in mouse model[J].Chinese Journal of Immunology,2011,27(9):779-782. |
| |
| Authors: | HE Xiang-Feng WANG Jing YU Fang-Liu ZHAO Feng-Shu CAI Kai ZHANG Hong-Yi CHEN Jun-Song CHEN Deng-Yu DOU Jun |
| |
| Affiliation: | HE Xiang-Feng,WANG Jing,YU Fang-Liu,ZHAO Feng-Shu,CAI Kai,ZHANG Hong-Yi,CHEN Jun-Song,CHEN Deng-Yu,DOU Jun.Department of Pathogenic Biology and Immunology,Medical School,Southeast University,Nanjing 210009,China |
| |
| Abstract: | Objective:The safety and anti-tumor efficacy of B16F10/ESAT-6-GPI-IL-21 tumour vaccine in mouse model was evaluated.Methods:Expressions of ESAT-6-GPI antigen of tumour vaccine were detected by immunofluorescence and flow cytometry,respectively.Expression of IL-21 of tumour vaccine were detected by Western blot.The biological safety of tumour vaccine was evaluated by animal experiments.The developed postoperative bearing-tumor mice were immunized with the tumour vaccine and the efficacy of B16F10/ESAT-6-GPI-... |
| |
| Keywords: | IL-21 ESAT-6 GPI |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
