川芎嗪对蛛网膜下腔出血后血浆及脑脊液中内皮素和一氧化氮含量的影响

目的观察蛛网膜下腔出血(SAH)后血浆和脑脊液内皮素(ET)、一氧化氮(NO)水平的变化及神经功能受损情况,探讨川芎嗪对SAH后脑血管痉挛(CVS)的防治作用。方法实验分为对照组、实验组和空白组,对照组和实验组采用枕大池注血法制作SAH模型。实验组经腹腔给予川芎嗪注射液,对照组和空白组仅给予生理盐水。用放射免疫法检测造模后72 h和168 h血浆和脑脊液ET和NO水平,并对动物进行神经功能评分。结果1SAH后,脑脊液中对照组的ET水平较实验组和空白组增加(P<0.05),且168 h水平高于72 h;血浆中对照组ET水平亦高于实验组和空白组(P<0.05),空白组和实验组之间差异无统计学意义。2SAH后,脑脊液中对照组NO水平较空白组和实验组低(P<0.05),3组的NO水平随着时间的推移变化不大;血浆中对照组NO水平亦较空白组和实验组低(P<0.05),空白组和实验组之间差异无统计学意义。3在神经功能评分方面,各时点上实验组评分均低于对照组(P<0.05)。结论采用川芎嗪治疗后,血浆和脑脊液中ET水平有所下降或ET上升受到抑制,而NO水平则有所上升或NO下降受到抑制,提示川芎嗪对SAH后CVS有较好的防治作用。

Effects of Tetramethylpyrazine on Endothelin and Nitric Oxide Contents in Plasma and Cerebrospinal Fluid after Subarachnoid Hemorrhage

OBJECTIVE: To observe the variation of endothelin (ET) and nitric oxide (NO) in plasma and cerebrospinal fluid (CSF) in rabbits, and evaluate the effects of tetramethylpyrazine (TMP) on the prevention and cure of cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH). METHODS: 24 New Zealand rabbits were randomly assigned into three groups: contrast group, experiment group and blank group. Every group contained 8 rabbits. SAH was established according to inject blood into the cisterna magna. The experiment group was administrated with TMP (20 mg/kg x d) transperitoneally. ET and NO of plasma and CSF were detected by radical immunoassay at 72 h and 168 h after SAH. Neurofunction were detected in every group at all the time scales. RESULTS: (1) After SAH, the level of ET in CSF increased significantly in contrast group compared with that in experiment and blank groups (P<0.05). The value of ET at 168 h was higher than that at 72 h. The level of ET in plasma increased significantly in contrast group compared with blank and experiment groups (P<0.05), and no significant contrast could be found between blank and experiment group. (2) After SAH, the value of NO in CSF was lower in contrast group than in other groups (P<0.05), and the level of NO in CSF continued to decrease in all groups on some extent. As time went by, no significant contrast could be found in all groups. The value of NO in plasma was lower in contrast group than in other groups (P<0.05). There was no significant difference between experiment and blank groups. (3) The neuro-function score continued to be increased in contrast group, but decreased in experiment one. The neuro-function score was lower in experiment group than in contrast one at every time point (P<0.05). CONCLUSION: After administration of TMP, the variation of ET has the continued decrease in plasma and CSF; the variation of NO shows the continued increase in plasma and CSF; neurological function gets possibly protected. TMP may prevent from and cure CVS after SAH.