气相色谱/质谱在不同种类硫酸盐还原菌的鉴定中应用探讨

采用气相色谱／质谱联用技术分析和鉴定吉林、长庆、胜利、新疆宝浪和江汉油田的硫酸盐还原菌（ｓｕｌｆａｔｅ－ｒｅｄｕｃｉｎｇ ｂａｃ６ｅｉａ，ＳＲＢ）代谢产物，利用透射电镜分析和观察五油田ＳＲＢ的形貌。结果表明，吉林、长庆、胜利、新疆宝浪ＳＲＢ呈杆状，培养基中的代谢产物主要以丙酸为主，江汉油田的ＳＲＢ呈弧状，培养基中的代谢产物主要以苯甲酸为主。该技术为油田提供了一种鉴定ＳＲＢ代谢产物判别其种类的快捷准

Identification of Various Sulfate Reducing Bateria Species by Gas Chromatography-Mass Spectrometry

The metabolites of sulfate reducing bacteria (SRB) from Jianghan, Jilin, Changqing, Shengli, and Xinjiang Oiffield have been identified by using the gas chromatography (GC) and mass spectrometric (MS) technique, Also, their microstructural charecterizations based on transmission electron microscopy (TEM) are analyzed. The results showed that SRB of five oilfields were different in size and microstructural characterization. The metabolites of SRB from five oiffields were not the same.The main metabolite of Jianghan's SRB was benzoic acid and from the other four Oiffields was propanoic acid. GC/MS technique is a rapid and accurate methed to identify the metabolite and species of SRB in the oiffield.