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| 木质纤维素酶高产菌株的筛选及其对玉米秸秆降解效果的影响 | |
| 引用本文： | 郭红伟,王娇,常娟,尹清强,左瑞雨,郑秋红,王平,王潇,刘俊熙.木质纤维素酶高产菌株的筛选及其对玉米秸秆降解效果的影响[J].生物加工过程,2012,10(5):39-43. |
| 作者姓名： | 郭红伟 王娇 常娟 尹清强 左瑞雨 郑秋红 王平 王潇 刘俊熙 |
| 作者单位： | 1. 河南农业大学牧医工程学院,郑州,450002 2. 河南农业大学牧医工程学院,郑州450002;河南省饲料微生物工程技术研究中心,周口466000 3. 河南省饲料微生物工程技术研究中心,周口,466000 |
| 摘 要： | 为了提高玉米秸秆中木质素的降解率,从腐烂的树枝和土壤中筛选木质纤维素酶高产菌株,以秸秆为唯一C源富集培养后,采用PDA-愈创木酚法进行初筛,筛选出产木质素酶真菌5株,然后以玉米秸秆为主要C源进行固态发酵和复筛。结果表明:第5号菌株在发酵玉米秸秆5 d后,使木质素的降解率达到最高(34.95%),粗纤维的降解率达到20.00%,显著高于其他4种菌株(P<0.05),其羧甲基纤维素酶比酶活达到116.35 U/g;10 d后,其木质素酶比酶活达到最高(45.64 U/g)。 |
| 关 键 词： | 木质素酶 玉米秸秆 降解率 |
Selection of ligninase-producing microbes and analysis of their effects on corn stalk degradation |
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| GUO Hongwei , WANG Jiao , CHANG Juan , YIN Qingqiang , ZUO Ruiyu , ZHENG Qiuhong , WANG Ping , WANG Xiao , LIU Junxi.Selection of ligninase-producing microbes and analysis of their effects on corn stalk degradation[J].Chinese Journal of Bioprocess Engineering,2012,10(5):39-43. | |
| Authors: | GUO Hongwei WANG Jiao CHANG Juan YIN Qingqiang ZUO Ruiyu ZHENG Qiuhong WANG Ping WANG Xiao LIU Junxi |
| Affiliation: | 1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Engineering ＆ Technology Research Center of Feed Microbes, Zhoukou, 466000, China) |
| Abstract: | In order to increase corn stalk degradation, five strains of ligninase-producing microbes were selected from rotten plants and soil with the guaiacol as the indicator. The results showed that the fifth strain could produce CMCase at 116.35 U/g, degradation rates of lignin and crude fiber reached 34.95% and 20.00% （P 〈 0.05 ） after 5 d solid-state fermentation. The ligninase activity after 10 d fer mentation medium reached 45.64 U/g. |
| Keywords: | ligninase corn stalk degradation rate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
