安徽省3种常见蜚蠊的可溶性蛋白质组成分析

目的 应用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（SDS－PAGE）对安徽省3种常见蜚蠊的可溶性蛋白质区带进行测定,为蜚蠊分类提供科学依据。方法 应用冻融匀浆法制备3种蜚蠊的可溶性蛋白液,进行SDS－PAGE,用考马斯亮蓝G－250染色后,观察、计算各蛋白质区带的分子量。结果 黑胸大蠊的蛋白条带数目♂为29条,♀为31条;美洲大蠊的蛋白条带数目♂为28条,♀为30条;德国小蠊的蛋白条带数目♂为25条,♀为28条。通过比较发现3种蜚蠊的蛋白质区带数目和位置有差别;德国小蠊主要蛋白质区带的益与两种大蠊相差明显,♀♂之间也有差别。结论SDS－PAGE可作为蜚蠊分类的手段之一。

Soluble protein analysis of three ordinary species of cockroaches in Anhui province

Objective To examine the soluble protein patterns of three ordinary species of cockroaches in Anhui province by SDS PAGE technique, and to find the method of scientific classification for cockroaches. Methods The soluble protein liquid was made by the way of freezing and melting homogenate. SDS PAGE was carried out in the liquid. After using Coomassie brilliant blue G 250 , the molecular weight of all of protein strips were measured and calculated by standard curve. Results The numbers of female and male of Periplaneta fuliginosa were 31 strips and 29 strips; the numbers of female and male of Periplaneta Americana were 30 strips and 28strips; the numbers of female and male of Blattella germarica were 28 strips and 25 strips. The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect. Conclusion SDS PAGE can be used as one of classification methods for cockroaches.