非小细胞肺癌淋巴结转移相关因素的研究

目的：检测尿激酶纤溶酶原激活物（uPA）、尿激酶纤溶酶原激活物抑制剂（PAI-1）、血管内皮生长因子（VEGF）和微m管密度（MVD）在非小细胞肺癌（NSCLC）组织中的表达以及与淋巴结转移的关系。方法：采用免疫组织化学方法联合检测52例NSCLC组织中uPA、PAI—1、VEGF和MVD的表达水平。结果：uPA、PAI—1、VEGF和MVD在NSCLC中的表达显著高于正常肺组织，影响淋巴结转移的相关因素是TNM分期（P〈0．001）、肿瘤侵犯程度（P=0．034）、uPA表达（P=0．048）、VEGF表达（P=0．047）。多因素分析表明VEGF高表达是淋巴结转移的独立影响因素（P=0．043）。结论：uPA、VEGF高表达与NSCLC的淋巴结转移密切相关，促进了肿瘤转移。

Related factors with lymph nodes metastasis in non-small cell lung cancer

Objective： Lymph nodes metastasis have focused on the relation between degradation of extracellular matrix, invasaion, migration,and tumor angiogenesis. The aim of this study is to evaluate the effects of the urokinase-type plasminogen aetivato （uPA） system and vascular endothelial cell growth factor（VEGF） on tumor invasion and lymph nodes metastasis in NSCLC. Meth2 otis ： Expression of uPA, plasminogen activator inhibitor（ PAI-1 ）, VEGF and microvessel density （MVD） was retrospectively determined by ABC immunohistochemical technique in 52 patients with NSCLC. Results：Tile positive rates of uPA （80. 8% ）, PAI-1 （80. 8% ） and VEGF（75.0% ） in NSCLC were respectively significantly higher than that in normal lung tisssues（P 〈0. 001 ）. A significant correlation of lymph nodes metastasis was found with uPA （ P 〈 0. 048 ） , VEGF （ P 〈 0. 047 ）, clinical stage （ P 〈 0. 001 ） and tumor invasion （P 〈 0. 034 ）, and multivariate regression analysis showed VEGF was the most risk factor. Conclusion ： Overexpression of uPA and VEGF may contribute to lymph nodes metastasis.