番茄含氮量快速检测微电极的研制 |
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引用本文: | 左志宇,骆飞,毛罕平,马万征,张晓东,李青林,秦丽娟,吕天远. 番茄含氮量快速检测微电极的研制[J]. 农机化研究, 2016, 0(3): 174-178. DOI: 10.3969/j.issn.1003-188X.2016.03.035 |
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作者姓名: | 左志宇 骆飞 毛罕平 马万征 张晓东 李青林 秦丽娟 吕天远 |
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作者单位: | 江苏大学现代农业装备与技术教育部重点实验室,江苏镇江,212013 |
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基金项目: | 国家自然科学基金,国家自然科学基金重点项目,“十二五”国家科技支撑计划项目,安徽省科技攻关计划项目 |
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摘 要: | 针对设施栽培番茄营养信息检测的需求,提出了一种基于多管离子选择性微电极的番茄含氮量快速检测方法。为此,详细讨论了多管离子选择性微电极的拉制、硅烷化、敏感剂充灌和标定等制备过程;通过对现有硝酸根离子和铵根离子敏感剂配方进行试验分析,筛选出了适用于多管离子选择性微电极的硝酸根离子和铵根离子敏感膜。通过番茄叶片样本测试,并与AA3连续流动分析仪测得的含氮量相比,采用多管离子选择性微电极测得的番茄叶片样本含氮量绝对误差为1.03mg/g,相对误差为3.96%。试验结果表明:采用多管硝酸根离子和铵根离子选择性微电极检测番茄叶片中的含氮量是可行的。该研究可为同时快速检测作物中钾、钙等离子的含量提供参考。
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关 键 词: | 番茄 含氮量 多管离子选择性微电极 硝酸根离子 铵根离子 |
Development of Rapid Detection Microelectrode of Tomato Nitrogen Content |
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Abstract: | To meet the requirement for nutrition information detection of tomato cultivated in protected agriculture , a method for rapid detection of tomato nitrogen content based on multibarrel ion selective microelectrode was proposed .The preparation process of drawn , silanization , sensitive agent filling , and calibration of multibarrel ion selective microelec-trode were discussed in detail .Through test and analysis of the present sensitive formulation of NO 3-and NH4+, the NO3-and NH4+sensitive membranes which are suitable for multibarrel ion selective microelectrode were screened .In comparison with the nitrogen content detected by AA 3 continuous flow analyzer , the absolute error and the relative error for the nitrogen content of the tomato leaf sample are 1 .03 mg/g and 3 .96%, respectively , through the test of tomato leaf sample by multibarrel ion selective microelectrode .The experimental results showed that:it is feasible to detect the nitro-gen content of tomato leaf by the multibarrel NO 3-ion and NH4+ion selective microelectrode .Besides, this study can provide a reference for detection of the content of potassium and calcium etc .of crops. |
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Keywords: | tomato nitrogen content multibarrel ion selective microelectrode NO3- NH4+ |
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