| 疏水层析在分离纯化里氏木霉重组t-PA中的应用 |
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| 引用本文： | 曹畅,冮洁,江成英. 疏水层析在分离纯化里氏木霉重组t-PA中的应用[J]. 齐齐哈尔大学学报(自然科学版), 2005, 21(1): 15-18 |
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| 作者姓名： | 曹畅 冮洁 江成英 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006 |
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| 摘 要： | 疏水层析是一种有效的分离纯化手段，本文采用Octyl Sepharose和Butyl Sepharose两种疏水层析填料对里氏木霉表达的t-PA的提纯操作条件进行了初步研究。结果表明，选用Butyl Sepharose FF填料，上样量10mL（蛋白含量4．1mg／mL)，用15％(NH4)2SO4的PBS以2mL／min的流速进行洗脱时，回收的酶的比活性较大，为2959．54U／mg，纯化倍数为5．16，回收率为7．12％。
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| 关 键 词： | 里氏木霉 t-PA 疏水层析 |
| 文章编号： | 1007-984X(2005)01-0015-04 |
| 修稿时间： | 2004-10-21 |
Hydrophobic interaction chromatography to the purification of t-PA produced by trichodera reesei |
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| GAO Chang,GANG Jie,JIANG Cheng-ying. Hydrophobic interaction chromatography to the purification of t-PA produced by trichodera reesei[J]. Journal of Qiqihar University(Natural Science Edition), 2005, 21(1): 15-18 |
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| Authors: | GAO Chang GANG Jie JIANG Cheng-ying |
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| Abstract: | Hydrophobic interaction chromatography is an effective method for isolation and purification.This article demonstrates the application of Hydrophobic interaction chromatography to the purification of t-PA produced by Trichoderma reesei and the optimization of separating and purificating conditions of t-PA. The purification operation conditions of Octyl Sepharose FF and Butyl Sepharose FF columns wrer primarily researched. The result showed that Butyl Sepharose FF was suitable for separation of t-PA. The optimized chromatography condition was start elution butter 0.02mul/L PBS(pH7.4) contained 15% (NH4)2SO4 at a rate of 2mL/min. The enzyme specific activity is 2959.54U/mg.The purification multiple is 5.16.The t-PA activity recovery is 7.12%. |
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| Keywords: | Trichoderma reesei t-PA hydrophobic interaction chromatography |
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