7种生物碱在不同粒径卷烟主流烟气气溶胶中的分布研究

建立了烟气气溶胶中7种生物碱的气相色谱-质谱(GC-MS)测定方法,并采用电子低压撞击器(ELPI)分12级捕集烟气气溶胶粒相物,研究了卷烟主流烟气气溶胶中7种生物碱含量和浓度的粒径分布。捕集的气溶胶样品加入氢氧化钠溶液和二氯甲烷进行碱法提取,提取液经DB-5MS弹性毛细管柱分离,选择离子监测模式测定,内标法定量。结果表明:该法检测主流烟气气溶胶中7种生物碱的相对标准偏差(RSD)为2.1%~6.4%,检出限为0.39~14.84 ng/cig,加标回收率为85.5%~124.8%。7种生物碱主要分布于0.144~0.722μm的中等粒径气溶胶粒相物中,在粒径0.431μm的粒相物中含量最高,与捕集的粒相物质量分布一致。7种生物碱在不同粒径气溶胶粒相物中的浓度基本趋于一致,其浓度随气溶胶粒径的分布无特异性。

Distribution of 7 Alkaloids in Aerosol of Various Particle Sizes from Mainstream Cigarette Smoke

A method was developed for the analysis of 7 alkaloids in mainstream cigarette smoke aerosol by gas chromatography-mass spectrometry.By using electrical low-pressure impactor (ELPI),particles of cigarette smoke aerosol were divided into 12 stages and collected.The content and concentration distributions of 7 alkaloids with respect to smoke aerosol particle size were investigated.The collected aerosol particles were treated by alkali extraction method with sodium hydroxide solution and dichloromethane.The extract was separated with a DB-5 MS capillary column under selected ion monitoring(SIM) mode.The targets were quantitatively analyzed using internal standard method.The results showed that,the relative standard deviations (RSDs) for the established method ranged from 2.1％ to 6.4％,with limits of detection(LODs) of 0.39-14.84 ng/cig,and the recoveries at three spiked levels ranged from 85.5％ to 124.8％.All of 7 alkaloids mainly distributed in aerosol of medium size particles(0.144-0.722 μm) with a peak content at particle size of 0.431 tμm,which was similar to the distribution pattern of collected particle mass.The concentrations of 7 alkaloids were uniform throughout the particles of various sizes,indicating a non-specificity distribution.