长期停服棉酚者精子功能的探讨

观察了19名停服棉酚3—10年的志愿者(实验组)及19名年龄相近具有生育能力的健康男子(对照组),根据Rogers 法略加改进的SPA 实验,实验组和对照组的受精百分率有显著差异(P<0.01),6例重复试验者的受精百分率十分接近。经Spearman 法试验,受精浓度与精子穿入百分率等级相关,有显著差异(r_s=0.94285,P<0.05),2×C 表线性回归显著性检验说明,不同受精浓度的穿入率有非常显著差别(X~2=80.6124,P<0.001),在10~7～10~4受精浓度范围穿透百分率依次递减,说明在这段范围内随着受精浓度的增加,穿入率有显著升高趋势。精子染色体异质性实验(SCHT)显示,二组差异不明显(P>0.05)。实验组精子的ATP 含量明显低于对照组(P<0.01)。SPA 受精百分率与ATP 含量相关(r=0.5984,P<0.05)。两组的LH 和T 水平无明显差异,而实验组的FSH 水平则明显高于对照(P<0.05)。结果表明,长期停服棉酚者精子的受精功能低于正常人,可能不是血中T 水平下降之故。

STUDIES ON SPERM FUNCTION IN MAN LONG AFTER CESSATION OF GOSSYPOL ADMINISTRATION

Obsered were 19 volunteers(treated group)who had stopped gossypol administration3 to 10 years and 19 healthy,fertile men(control group)of similar age.Sperm penetrationassay(SPA)showed that the difference between the penetration rates of the treated andcontrol was remarkable(P<0.01).The penetration rates in the repetition of 6 cases were quite similar to each other.By Spearman rank correlation method,it was found that therewas a significant difference between sperm density of insemination and sperm penetrationrates(r_s=0.94258,P<0.05).The test of 2 X C linear regression also showed there wasa significant correlation between the penetration rates and the insemination density(X~2=80.6124,P<0.001).Sperm chromatin heterogeneity test(SCHT)showed no difference(P>0.05)between both groups.Sperm ATP contents of the treated was notably lowerthan that of the control(P<0.01).There was a correlation between the penetration ratesand ATP contents of sperm(r=0.5984,P<0.05).There was significant differencebetween the LH and T level in blood of the two groups,while FSH of the treated was higherthan that of the control(P<0.05).The results indicate that the sperm fertilization function of man long afetr cessation ofgossypol treatment was lower than that of the normal,which might be due to reasons otherthan the decrease in blood T level.