银杏叶提取物对糖尿病大鼠学习记忆障碍的影响

目的探讨银杏叶提取物(EGb)对链脲佐菌素(STZ)诱导的糖尿病大鼠学习记忆障碍的影响。方法利用STZ(48 mg·kg~(-1))制备大鼠糖尿病模型,将糖尿病大鼠随机分为模型组、胰岛素组(胰岛素10 U·kg~(-1)皮下注射)、EGb高剂量组(EGb 100 mg·kg~(-1)灌胃给药)和低剂量组(EGb 50 mg·kg~(-1)灌胃给药),另取大鼠腹腔注射pH 4.4柠檬酸缓冲液作为对照组,每组15只。给药3个月后,Morris水迷宫观察各组大鼠行为学差别。结果 Morris水迷宫结果表明,与对照组相比,糖尿病大鼠在训练后前6日平均值及第7天第1次穿越目标区域的时间延长、穿越目标区域的次数明显减少、在第1象限的时间和路程也明显缩短(P<0.01);与模型组大鼠相比,胰岛素组和EGb高、低剂量组大鼠第1次穿越目标区域的时间明显缩短、穿越目标区域的次数明显增多、在第1象限的时间和路程明显延长(P<0.01);EGb高、低剂量组大鼠在第7天第1次穿越目标区域的时间明显长于胰岛素组(P<0.05);其余指标间比较无统计学差别(P>0.05)。结论银杏叶提取物对糖尿病大鼠学习记忆障碍有明显改善作用。

Effects of Gingko leaf extractive on learning and memory in the diabetic rats

Objective To explore the effect of extract of Gingko biloba leaves(EGb) on the streptozotocin(STZ)-induced diabetic rats with learning and memory impairment.Methods The diabetic rats model were established by injection of STZ (48 mg·kg~(-1)).Then the rats were randomly divided into model group,insulin treatment group(insulin 10 U·kg~(-1),hypodermic injection),high dose EGb group(EGb 100 mg·kg~(-1),intragastric administration) and low dose EGb group(EGb 50 mg·kg~(-1),intragastric administration),fifteen rats in each group.Fifteen rats in control group were treated with citrate buffer solution by intraperitoneal injection.After 3 months of administration,the ethological changes of learning and memory were observed through Morris water maze test.Results Compared with control group,the results of Morris water maze test showed that, for the diabetic rats,the times to firstly cross the target area from the first to the sixth day and the seventh day after training both prolonged,the frequencies to cross the target area also decreased significantly(P<0.01),the time and distance in the first quadrant also decreased significantly(P<0.01).Compared with the rats in diabetic model group,for the rats in the insulin and the EGb treatment groups(high and low dose),the time to firstly cross the target area was shortened significantly,the frequencies to cross target area increased significantly and the scores of time and distance in the first quadrant also increased significantly (P<0.01);The times to firstly cross the target area at the seventh day in the EGb treatment group(high and low dose) were longer than that in the insulin treatment group(P<0.05);And there was no statistical significance among the other groups(P>0.05).Conclusion EGb can significantly ameliorate the deficiency of learning and memory induced by diabetes.