| 南蛇藤素抑制豚鼠体外精子的受精能力 |
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| 引用本文： | 袁玉英,顾芝萍,石其贤,秦国伟,徐任生,曹霖.南蛇藤素抑制豚鼠体外精子的受精能力[J].药学学报,1995,30(5):331-335. |
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| 作者姓名： | 袁玉英 顾芝萍 石其贤 秦国伟 徐任生 曹霖 |
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| 作者单位： | 浙江省医学科学院，中国科学院上海药物研究所 |
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| 摘 要： | 南蛇藤素(Cel)对豚鼠精子前向运动(FM)、获能(Cap)、顶体反应(AR)和穿透去透明带仓鼠卵(SPA)均有明显的抑制作用,其作用强度随剂量而增加;对Cel的敏感性依次为精子Cap>FM>SPA>AR。Cel对豚鼠精子AR,FM和Cap的抑制作用比乙酸棉酚(GA)明显为强。
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| 关 键 词： | 南蛇藤素 精子 获能 顶体反应 精卵相互作用 |
| 收稿时间： | 1994-08-30 |
IN VITRO INHIBITION OF CELASTROL ON SPERMATOZOA FERTILIZATION ABILITY OF GUINEA PIG |
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| YY Yuan,ZP Gu,QX Shi,GW Qin,RS Xu and L Cao.IN VITRO INHIBITION OF CELASTROL ON SPERMATOZOA FERTILIZATION ABILITY OF GUINEA PIG[J].Acta Pharmaceutica Sinica,1995,30(5):331-335. |
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| Authors: | YY Yuan ZP Gu QX Shi GW Qin RS Xu and L Cao |
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| Abstract: | The effects of celastrol(Cel),isolated from Tripterygium wilfordii,on guinea pigsperm forward motility(FM),capacitation(Cap),the acrosome reaction(AR)and spermpenetration assay(SPA)were assessed in vitro.Cel(5 μg· ml-1)was found to inhibit thesespermatozoal functions,and the inhibitions were proportional to the concentrations of Cel used.Thepotency of inhibition of Cel on the fertilizing ability in guinea pig spermatozoa in vitro seems to followthe order :Cap>FM>SPA>AR.The inhibitory effect appeared to be reversible after washing awayCel if the duration of exposure of spermatozoa to Cel was shorter than 3 h. In a comparative study,the inhibitory effects of Cel on guinea pig sperm FM and AR were significantly stronger than those ofgossypol acetic acid. |
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| Keywords: | Celastrol Capacitation Sperm Acrosome reaction Sperm penetration assay |
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