海带多糖对人肝癌细胞Bel-7402增殖的抑制作用及其机制

目的：探讨不同质量浓度的海带多糖对人肝癌细胞Bel-7402增殖的抑制作用。方法：分别以质量浓度为5、10、20、30 mg/mL的海带多糖处理Bel-7402细胞，48 h后观察海带多糖对细胞增殖的抑制情况，Annexin V-FITC试剂盒检测细胞凋亡状况，酶联免疫吸附（enzyme-linked immunosorbent assay，ELISA）法检测Caspase-3活性，Western blotting法检测细胞内Caspase-3和甲胎球蛋白（alpha-fetoprotein，AFP）表达量。结果：海带多糖对Bel-7402细胞的增殖有明显抑制作用，最佳抑制剂量为30 mg/mL；随着海带多糖质量浓度的升高，细胞凋亡率明显增加（P＜0.05），海带多糖处理组Bel-7402细胞内的Caspase-3表达水平同正常对照组细胞相比呈上升趋势，差异具有统计学意义（P＜0.05）；AFP蛋白表达水平呈下降趋势。结论：海带多糖对人肝癌细胞Bel-7402增殖具有抑制作用，其机制可能是激活Caspase-3，引起细胞凋亡和AFP蛋白表达水平下降所致。

Inhibitory Effect and Mechanism of Laminarin on Proliferation of Human Hepatoma Carcinoma Bel-7402 Cells

Objective: To examine the inhibitory effect of different concentrations of laminarin on proliferation of human
hepatoma carcinoma Bel-7402 cells. Methods: Bel-7402 cells were cultured with different concentrations of laminarin for
48 h. The inhibitory effect of laminarin on the proliferation of Bel-7402 cells was evaluated by MTT (methyl thiazolyl
tetrazolium) assay. Cell apoptosis was detected using Annexin V-FITC Kit. The activity of Caspase-3 was examined by
enzyme-linked immunosorbent assay (ELISA). The expression of Caspase-3 and alpha-fetoprotein (AFP) was detected
by Western blotting. Results: Laminarin inhibited the proliferation of Bel-7402 cells in a concentration-dependent manner
with an optimal concentration of 30 mg/mL. The increased concentration of laminarin could accelerate the apoptosis rate of
cells (P < 0. 05). Caspase-3 activity significantly increased in the treatment groups when compared with the control group
(P < 0.05). The expression of Caspase-3 increased significantly and AFP expression decreased significantly in the treatment
groups. Conclusion: Laminarin can inhibit the proliferation of Bel-7402 cells, and the mechanism may be associated with
cell apoptosis caused by the activation of Caspase-3, and reduced expression of AFP.