|
|||||
|
|
| CdTe量子点免疫层析试纸条检测克伦特罗 | |
| 引用本文： | 张金艳,熊 艳,廖且根,李伟红,王冬根,张 莉,罗林广. CdTe量子点免疫层析试纸条检测克伦特罗[J]. 食品科学, 2015, 36(22): 161-164. DOI: 10.7506/spkx1002-6630-201522030 |
| 作者姓名： | 张金艳 熊 艳 廖且根 李伟红 王冬根 张 莉 罗林广 |
| 作者单位： | 江西省农业科学院农产品质量安全与标准研究所，农产品质量安全重点实验室，农业部畜禽产品质量安全风险评估实验室（南昌），江西 南昌 330200 |
| 基金项目： | 江西省农业科学院创新基金（青年基金）项目（2012CQN007）；2015年度国家农产品质量安全风险评估项目（GJFP201500801） |
| 摘 要： | 以巯基乙酸作为稳定剂，利用水热反应制备了水溶性的高荧光CdTe量子点，量子产率为54.961%。研究了该量子点在1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐和N-羟基丁二酰亚胺的作用下与抗克伦特罗抗体的共价连接。结果表明：该量子点与克伦特罗抗体连接后体系的荧光强度和吸光度均增强，且峰位均未发生明显移动，F/P值为4.20。将该偶联反应物CdTe-Anti CLE pAb作为荧光标记物，组装出一种用于检测克伦特罗的荧光免疫试纸条，最低检测限达0.5 μg/L，与酶联免疫吸附法进行对比，该试纸条能够实现对克伦特罗的快速检测。 |
| 关 键 词： | 水热合成 CdTe量子点 克伦特罗 免疫层析试纸条 |
Development of CdTe Quantum Dots-Based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol |
|
| ZHANG Jinyan,XIONG Yan,LIAO Qiegen,LI Weihong,WANG Donggen,ZHANG Li,LUO Linguang. Development of CdTe Quantum Dots-Based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol[J]. Food Science, 2015, 36(22): 161-164. DOI: 10.7506/spkx1002-6630-201522030 | |
| Authors: | ZHANG Jinyan XIONG Yan LIAO Qiegen LI Weihong WANG Donggen ZHANG Li LUO Linguang |
| Affiliation: | Laboratory of Quality and Safety Risk Assessment for Livestock and Poultry Products (Nanchang),Ministry of Agriculture, Key Laboratory of Quality and Safety of Agricultural Products, Institute for Quality and Safety and Standards ofAgricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China |
| Abstract: | CdTe quantum dots (QDs) were synthesized in aqueous solution by using aercapto acetic acid as the stabilizer, and then coupled to the polyclonal antibody (Anti-Clenbuterol pAb, Anti-CLE pAb) with N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). Spectral analysis further showed that the photoluminescence (PL) intensity and absorbance value of the CdTe-Anti-CLE pAb were enhanced without significant shifts in the characteristic peaks. Using the CdTe-Anti-CLE pAb, an immunochromatographic test strip was developed for the rapid detection of clenbuterol (CLE) with a detection limit of 0.5 μg/L. Compared with the results of enzymelinked immunosorbent assay (ELISA), the lateral flow test strip is very useful and effective as a rapid screening method for detection of CLE residues. |
| Keywords: | hydrothermal synthesis CdTe quantum dots clenbuterol immunochromatographic test strip |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《食品科学》浏览原始摘要信息 | |
| 点击此处可从《食品科学》下载全文 | |
