人乳头瘤病毒基因型与宫颈病变关系的研究

目的:了解人乳头瘤病毒(HPV)感染基因型分布,探讨不同HPV基因型感染与宫颈病变的关系。方法:收集门诊就诊者宫颈脱落细胞行液基薄层细胞检测并按照TBS分类标准进行细胞学诊断,同时应用低密度基因芯片技术进行HPV检测和基因型分析(15种高危基因型、5种低危基因型和Cp8304基因)。结果:862例标本中,共检出19种HPV基因型。宫颈细胞学正常(NILM)组前3位HPV基因型是HPV11、6和42,HPV单一与多重感染分别占75.86%和24.14%;细胞学异常组居前3位的HPV基因型是HPV16、52和58,HPV单一与多重感染分别占71.11%和28.89%,该组以高危型HPV感染为主(88.71%)。细胞学正常组和异常组HPV感染率分别是11.28%和64.66%(χ2=299.33,P<0.05)。随宫颈病变级别递增,HPV感染率呈增高趋势。结论:不同的HPV基因型具有不同的致病力,HPV多重感染与宫颈病变的发生、发展密切相关,HPV感染率随宫颈病变程度增加而增加。

The study of relationship between human papillomavirus genotypes and cervical epithelial lesions

Objective ： To investigate the cervical condition and genotypes of human papillomavirus （HPV） infection and explore the relationship between different genotypes of HPV infections and cervical epithelial lesions. Methods ： Cervical exfolia- ted cells were collected from 862 outpatients and examined by thin - cytologic test, and the cervical cytological conditions were evaluated by The Bethesda System （TBS） as negative for intraepithelial lesion or malignancy （（NILM） or different degrees of lesions. Low - density gene chip - based detection system for HPV genotypes （ 15 types of high - risk HPVs, 5 types of low - risk HPVs and CP8304 was employed to detect the HPV infections. Result： 19 of 21 HPV genotypes were detected in 862 specimens. The most common HPV genotypes were HPV11, 6, 42 and 16 in NILM group, and HPV16,52 and 58 in cervical lesion group. The detection rate of HPVs in NILM and lesion group were 11.28% and 64.66%, respectively. The constituent ratios of single and multiple infections of HPV genotypes were 75.86% and 24.14% in NILM group, and 71. 11% and 28. 89% in lesion group, respectively. The high -risk HPV genotypes were the most prevalent HPV infections （88.71%） in lesion group. The distribution of different HPV genotypes varied with the different degrees of cervical lesions. Conclusion： There was a close relationship between the multiple infection of HPV genotypes and the development of cervical epithelial lesions. Infection of HPV （s） increased with the grade of cervical lesions.