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Functionalized electrospun mats from styrene-maleic anhydride copolymers for immobilization of acetylcholinesterase |
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| Authors: | O. Stoilova N. Manolova T. Godjevargova I. Rashkov |
| Affiliation: | a Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., 103A, BG-1113 Sofia, Bulgaria b Department of Biotechnology, University “Prof. Dr. Asen Zlatarov”, Prof. Y. Yakimov St., 1, BG-8010 Burgas, Bulgaria c Institute of Genetics and Biophysics of CNR, Via Pietro Castellino, 111-80131 Naples, Italy d National Institute of Biosystems and Biostructures (INBB), Via le Medaglie d’Oro, 305, 00136 Rome, Italy |
| Abstract: | A series of electrospun mats of styrene-maleic anhydride copolymers has been functionalized by modification with two types of spacers - a polymer with a flexible hydrophilic polyether chain (Jeffamine® ED) and a rigid low-molecular-weight spacer (р-phenylenediamine). The successful modification of the microfibrous mats with these bifunctional compounds has been proved by ATR-FTIR and X-ray photoelectron spectroscopy (XPS) analyses. In a further step, acetylcholinesterase (AChE) has been covalently immobilized onto the modified mats using glutaraldehyde as a binding agent. The amount of bound protein and the specific activity of the immobilized AChE have been determined. The highest activity has been displayed by AChE covalently bound to Jeffamine-modified microfibrous mats. Moreover, the immobilized AChE is characterized by enhanced thermal and storage stability as compared to the free enzyme. |
| Keywords: | Electrospinning Enzyme immobilization Acetylcholinesterase Styrene-maleic anhydride copolymers Jeffamine |
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