| 青蒿琥酯对人白血病K562 细胞凋亡抑制蛋白表达的影响 |
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| 引用本文： | 王玮琴,王永钢,金晓红. 青蒿琥酯对人白血病K562 细胞凋亡抑制蛋白表达的影响[J]. 科学通报, 2011, 56(11): 828-831. DOI: 10.1360/972010-2003 |
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| 作者姓名： | 王玮琴 王永钢 金晓红 |
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| 作者单位： | 浙江省中医院药剂科;绍兴市中医院药剂科; |
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| 摘 要： | 用Western blot 法检测青蒿琥酯对白血病K562 细胞的X 染色体连锁凋亡抑制因子 (X-linked inhibitor of apoptosis protein, XIAP)、存活素(Survivin)、细胞凋亡抑制蛋白1 (cellular IAP1, cIAP-1)和细胞凋亡抑制蛋白2 (cellular IAP2, cIAP-2)表达的影响, 以及对K562 细胞半胱 氨酸天冬氨酸酶3 (caspase-3)和多聚ADP-核糖聚合酶裂解片段(poly ADP-ribose polymerase cleavage, PARP cleavage)的作用. 青蒿琥酯能够下调XIAP, Survivin 和cIAP-2 的表达, 上调 caspase-3 活化亚基(17 kD)和PARP cleavage (85 kD)的表达. 青蒿琥酯通过下调XIAP, Survivin 和cIAP-2 的表达来诱导白血病细胞凋亡, caspase-3 也与诱导白血病细胞凋亡有关.
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| 关 键 词： | 青蒿琥酯 IAP 家族 凋亡 caspase-3 PARP cleavage |
| 收稿时间： | 2011-01-14 |
Effects of Artesunate on the expression of inhibitor of apoptosis proteins in human erythroleukemia K562 cells |
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| WANG WeiQin,WANG YongGang , JIN XiaoHong. Effects of Artesunate on the expression of inhibitor of apoptosis proteins in human erythroleukemia K562 cells[J]. Chinese Science Bulletin, 2011, 56(11): 828-831. DOI: 10.1360/972010-2003 |
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| Authors: | WANG WeiQin WANG YongGang & JIN XiaoHong |
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| Affiliation: | WANG WeiQin1,WANG YongGang2 & JIN XiaoHong2 1 Department of Pharmacy,Zhejiang Provincial Hospital of Traditional Chinese Medicine,Hangzhou 310006,China,2 Department of Pharmacy,Traditional Chinese Medicine Hospital of Shaoxing City,Shaoxing 312000 |
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| Abstract: | Western blot was performed to detect the influence of Artesunate on the expression of X-linked inhibitor of apoptosis protein (XIAP), survivin, cellular IAP1 (cIAP-1), and cellular IAP2 (cIAP-2), and to investigate the effect of Artesunate on caspase-3 and poly ADP-ribose polymerase (PARP) cleavage in human erythroleukemia K562 cells. Artesunate downregulated the expression of XIAP, survivin, and cIAP-2, and upregulated the expression of a 17-kD caspase-3 cleavage fragment and an 85-kD PARP cleavage fragmen... |
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| Keywords: | Artesunate IAPs apoptosis caspase 3 PARP cleavage |
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