| STUDY ON BURDOCK STUNT VIROID (BSV)——THE DENATURING BEHAVIOR AND OCCURRENCE IN SEPARATE PLANT OF BSV RNA-1 AND RNA-2 |
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| 引用本文： | 陈炜,田波,杨希才,朱玉香,孙国栋.STUDY ON BURDOCK STUNT VIROID (BSV)——THE DENATURING BEHAVIOR AND OCCURRENCE IN SEPARATE PLANT OF BSV RNA-1 AND RNA-2[J].中国科学B辑(英文版),1986(2). |
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| 作者姓名： | 陈炜 田波 杨希才 朱玉香 孙国栋 |
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| 作者单位： | Institute of Microbiology，Academia Sinica，Beijing，Institute of Microbiology，Academia Sinica，Beijing，Institute of Microbiology，Academia Sinica，Beijing，Institute of Medicinal Plant Decelopment，Chinese Academy of Medical Sciences，Beijing，Institute of Medicinal Plant Decelopment，Chinese Academy of Medical Sciences，Beijing |
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| 摘 要： | By two-dimensional electrophoretic technique, it was confirmed that BSV RNA-1 and RNA-2 contained circular molecules and that they Were more stable than the ether viroids in thermodynamics. BSV RNAs extracted from individual diseased plants gave only one specific band on analytical polyacrylamide gel, either BSV RNA-1 or RNA-2. No plant which has yet been detected contained both. No variation whatever was observed in the electrophoresis mobility, even over a period of two and a half years for BSV RNA-1 and RNA-2.The calli developed from diseased leaves grew rather slowly compared with healthy ones. The replication of BSV RNA-1 in calli which had continuously grown for 6—8 months was evidenced by the distinct band of BSV RNA-1 on file gels.
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