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| 量子点标记技术同步检测抗弓形虫IgG和IgM抗体的实验研究 | |
| 引用本文： | 李玲,刘琳琳,白晓,罗慧琴,李蒙,蒲晓允.量子点标记技术同步检测抗弓形虫IgG和IgM抗体的实验研究[J].国际检验医学杂志,2011,32(17):1929-1931. |
| 作者姓名： | 李玲 刘琳琳 白晓 罗慧琴 李蒙 蒲晓允 |
| 作者单位： | 1. 中国人民解放军二六四医院检验科,太原,030001 2. 第三军医大学新桥医院检验科,重庆,400037 3. 武警陕西省总队医院检验科,西安,710054 |
| 基金项目： | 重大传染病专项基金资助项目 |
| 摘 要： | 目的建立抗弓形虫(TOX)IgG和IgM抗体同步检测体系。方法分别用不同发射波长的量子点标记羊抗人IgG、羊抗人IgM作为检测抗体,以免疫磁珠作为固相载体,建立抗弓形虫IgG和IgM抗体同步检测体系,并对该检测体系进行了优化;同时检测280份临床血清标本,并与进口酶联免疫吸附测定(ELISA)试剂盒进行方法学比较。结果 TOX抗原的最适固化浓度为50μg/mL,固化效率在35%～55%之间。抗TOX IgG、IgM抗体检测结果与进口ELISA试剂盒相比符合率分别为96.4%,97.1%,本体系同时检测两种抗体的阳性检测率达8.2%。结论成功建立了抗TOX IgG和IgM抗体同步检测体系,该体系操作简便快速,灵敏度高,具有有良好的临床应用价值。 |
| 关 键 词： | 量子点 抗弓形虫IgG抗体 抗弓形虫IgM抗体 同步检测 |
Synchronous detection of anti-TOX IgG and IgM based on quantum dot technique |
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| Li Ling,Liu Linlin,Bai Xiao,Luo Huiqin,Li Meng,Pu Xiaoyun.Synchronous detection of anti-TOX IgG and IgM based on quantum dot technique[J].International Journal of Laboratory Medicine,2011,32(17):1929-1931. | |
| Authors: | Li Ling Liu Linlin Bai Xiao Luo Huiqin Li Meng Pu Xiaoyun |
| Affiliation: | 1.Department of Laboratory Medicine,Chinese People′s Liberation Army 264th Hospital,Taiyuan 030001,China; 2.Department of Laboratory Medicine,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China; 3.Department of Laboratory Medicine,Shanxi People′s Armed Police Forces Hospital,Xi′an 710054,China) |
| Abstract: | Objective To construct the synchronous detection system of anti-Toxoplasma(TOX) IgG and IgM antibodies.Methods Goat anti-human IgG and IgM antibodies,labeled with quantum dots of different emission wavelength,were used as detection antibody,and immunomagnetic beads were used as solid phase,to construct the synchronous detection system.280 cases of clinical serum samples were detected by optimized system and the results were compared with imported enzyme linked immunosorbent assay(ELISA) kit.Results The opti... |
| Keywords: | quantum dots anti-TOX IgG anti-TOX IgM simultaneous detection |
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