| HPLC法测定联磺甲氧苄啶片的含量 |
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| 引用本文： | 魏京京,张启明,金少鸿.HPLC法测定联磺甲氧苄啶片的含量[J].中国药事,2009,23(6):565-567. |
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| 作者姓名： | 魏京京 张启明 金少鸿 |
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| 作者单位： | 1. 中国药品生物制品检定所;中国医学科学院协和医科大学研究生院
2. 中国药品生物制品检定所 |
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| 摘 要： | 目的建立了同时测定联磺甲氧苄啶片中3个组分含量的高效液相色谱法。方法色谱柱为DikmaDiamonsil C18;流动相为甲醇-乙酸铵溶液（33∶67）,检测波长为210nm;流速为1mL.min-1。结果磺胺甲噁唑、磺胺嘧啶和甲氧苄啶的线性范围分别为50~150μg.mL-1（r=1.000）、50~150μg.mL-1（r=0.9999）、10~30μg.mL-1（r=0.9998）;平均回收率分别为101.6%（RSD=1.20%,n=9）、98.4%（RSD=0.95%,n=9）、98.7%（RSD=1.03%,n=9）。结论本法简单、快速、准确,可用于联磺甲氧苄啶片的定量分析。
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| 关 键 词： | 联磺甲氧苄啶片 磺胺甲噁唑 磺胺嘧啶 甲氧苄啶 高效液相色谱法 含量测定 |
Determination of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets by HPLC |
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| Wei Jingjing,Zhang Qiming,Jin Shaohong.Determination of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets by HPLC[J].Chinese Pharmaceutical Affairs,2009,23(6):565-567. |
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| Authors: | Wei Jingjing Zhang Qiming Jin Shaohong |
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| Affiliation: | Wei Jingjing, Zhang Qiming and Jin Shaohong(1 National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050;2 Graduate School of Chinese Academy of Medical Sciences and Peking Union Medical College) |
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| Abstract: | Objective A HPLC method was established to determination of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets. Methods Stationary phase was Dikma Diamonsil C18, mobile phase was methanol-ammonium acetate solution （33 ： 67）, the flow rate was 1mL · min^-1. Results There was a good linear relationship when the content of sulfamethoxazole, sulfadiazine and trimethoprim were within the range of 50-150μg · mL^-1（r= 1.000）, 50-150μg · mL^-1 （r=0. 9999） and 10-30μg · mL^-1 （r=0. 9998） respectively. The average recovery were 101.6%（RSD=1. 20%,n=9）, 98.4%（RSD=0.95%,n= 9） and 98. 7%（RSD=1.03% ,n=9） respectively. Conclusions This method is simple, rapid and accurate. It can be used for the quality control of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets. |
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| Keywords: | Sulfamethoxazole Sulfadiazine and Trimethoprim Tablets sulfamethoxazole sulfadiazine trimethoprim HPLC determination |
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