| 不同剂量链脲佐菌素建立妊娠期糖尿病大鼠模型稳定性的比较 |
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| 引用本文： | 李佳,魏波,塔娜,陈必良.不同剂量链脲佐菌素建立妊娠期糖尿病大鼠模型稳定性的比较[J].第四军医大学学报,2009(22):2500-2502. |
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| 作者姓名： | 李佳 魏波 塔娜 陈必良 |
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| 作者单位： | 第四军医大学西京医院妇产科; |
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| 摘 要： | 目的：探讨链脲佐菌素（STZ）建立妊娠期糖尿病（GDM）大鼠模型中不同给药剂量对模型稳定性的影响,研究GDM对母体及胎儿的影响率、转阴率和死亡率.方法：将46只SD孕鼠随机分为STZ低、中、高不同剂量组,分别一次性腹腔注射STZ35,45,60mg/kg;对照组腹腔注射等量的柠檬酸缓冲液.于妊娠第3,9,14,19d时测空腹血糖,并观察成模孕鼠在妊娠中体质量、饮水量、摄食量和尿量变化,比较孕鼠成模情况.结果：STZ45mg/kg组孕鼠在用药后出现明显的“三多一少”症状,其成模率83.3%为最高,转阴率最低.空腹血糖值STZ45mg/kg组（21.8±3.0）mmol/L与对照组（5.9±1.2）mmol/L相比较,其高血糖状态持续时间长,最稳定,且体质量也明显降低,差异具有统计学意义（P〈0.05）.结论：STZ45mg/kg腹腔注射给药是建立GDM大鼠模型的最佳剂量,具有较好的稳定性.
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| 关 键 词： | 妊娠期糖尿病 链脲佐菌素 模型 动物 |
Comparison of different dosage of streptozotocin-induced gestational diabetes mellitus on rats |
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| LI Jia,WEI Bo,TA Na,CHEN Bi-Liang.Comparison of different dosage of streptozotocin-induced gestational diabetes mellitus on rats[J].Journal of the Fourth Military Medical University,2009(22):2500-2502. |
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| Authors: | LI Jia WEI Bo TA Na CHEN Bi-Liang |
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| Affiliation: | LI Jia,WEI Bo,TA Na,CHEN Bi-Liang Department of Gynecology and Obstetrics,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM:To explore the effect of the stability of the rat model of gestational diabetes mellitus(GDM)by different dosage of streptozocin(STZ),for the research on maternal and fetal GDM provide experimental basis.METHODS:Forty-six Sprague-Dawley pregnant rats were selected and randomly assigned into 4 groups(the low doses group,the medium doses group,the high doses group and the control group).The rats were injected respec-tively with STZ(35,45,60 mg/kg)or equal dosage of citrate buffer solution intraperitoneall... |
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| Keywords: | gestational diabetes mellitus streptozotocin model animal |
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