虎杖化学成分研究

目的研究虎杖(Polygonum cuspidatum Sieb. et Zucc)的化学成分。方法实验分别以石油醚-乙酸乙酯(9∶1、7∶1、5∶1、3∶1、1∶1)作为硅胶柱色谱梯度洗脱剂,收集到的组分经制备HPLC进一步分离纯化,¹H-NMR、¹³C-NMR、DEPT等核磁共振谱和质谱的方法对化合物进行结构鉴定。结果分离并鉴定出8种化合物,分别是:22(Z)-麦角甾-4,6,8,22-四烯-3-酮(1)、(22E,24R)-豆甾-1,4-二烯-3-酮(2)、(E)-乙基十八碳-16-烯酸乙酯(3)、齐墩果酸(4)、大黄素(5)、白藜芦醇(6)、白藜芦醇苷(7)和6’-没食子酸-4-O-D-白藜芦醇苷酯(8)。结论化合物(1) 22(Z)-麦角甾-4,6,8,22-四烯-3-酮为新的天然产物(专利号:ZL 2013 10205435. 5),化合物(2)及(3)为虎杖药材中首次发现。

Chemical Constituents from Polygonum cuspidatum

OBJECTIVE To study the chemical constituents from Polygonum cuspidatum. METHODS The petroleum etherethyl acetate solvents at different ratios of 9∶ 1,7∶ 1,5∶ 1,3∶ 1 and 1∶ 1 were used as gradient eluents for silica gel column chromatography,and samples collected were further isolated and purified by preparative high performance liquid chromatography. Their structures were elucidated by physico-chemical constants and spectroscopic methods. RESULTS Eight compounds were obtained and identified as 22( Z)-ergosterol-4,6,8,22-tetraene-3-one( 1),( 22E,24R)-stigma-1,4-diene-3-one( 2),( E)-ethyl octadecanoic-16-enoic acid ethyl ester( 3),oleanolic acid( 4),emodin( 5),resveratrol( 6),trans-resveratrol glycoside( 7) and 6’-gallic acid-4-O-D-resveratrol ester( 8). CONCLUSION Compound 1 is a new natural compound( patent No. ZL 2013 10205435. 5),compound 2 and 3 are isolated from this plant for the first time.