Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications |
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| Affiliation: | 1. Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Materials Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China;2. Department of Chemistry, Tsinghua University, Beijing 100084, China;3. Tianjin Key Laboratory of Artificial Cell, Tianjin Third Central Hospital, Tianjin 300170, China;1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China;2. Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Beibei, Chongqing 400715, China;3. College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China;4. College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, China |
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| Abstract: | In the present research effort, production of crude sericin extracts from Bombyx mori silk cocoons was attempted using two different approaches. Sericin was extracted from cocoons by high-temperature autoclaving followed either by lyophilization or freezing-thawing precipitation, to obtain a crude sericin powder. The physico-chemical and biological characteristics of the crude sericin extracts were evaluated in detail, via FTIR, XRD, XRF, XRT, UV–vis scanning, TGA and DSC, protein quantification, antimicrobial activity, free radical scavenging activity, cytotoxic activity, potential for inducing chromosomal aberrations via Allium cepa assays, and genotoxicity via Comet™ analyses. The molecular weight distribution of the crude sericin extracts was also investigated, via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), and the results duly compared to standard sericin. The results gathered clearly suggest that the crude sericin extracts had both obvious radical scavenging effects with the 2.2-diphenyl-1-picryl-hydrazil (DPPH) assay, and antibacterial activity, further suggesting that this protein might be a valuable addition for either food and biopharmaceutical applications. |
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| Keywords: | Sericin Extraction Antimicrobial/antioxidant activity Cyto/genotoxicity Physico-chemical characterization |
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