CH50多肽真核表达载体pCH510的构建、表达及体内趋化和抑瘤作用

目的：探讨血管内皮生长因子的表达与肿瘤血管生成的关系。方法：将VEGF165正、反义RNA表达载体导入人胃癌细胞，观察接种VEGF高表达和低表达胃癌细胞裸鼠移植瘤的生长情况，并对移植瘤进行组织学检查，检测其血管密度、组织增生及环死程度等变化。结果：VEGF正义转染细胞所致移植瘤的生长速度明显快于反义转染细胞所致的移植瘤；组织学检查发现，正义转染细胞移植瘤的血管密度显著高于的转染细胞所致的肿瘤。结论：血管皮生长因子通过启动血管生成而促进肿瘤的生长，阻断血管内皮生长因子的产生可以抑制肿瘤的生长。

Construction and Expression of Eukaryotic Expressing Vector pCH510 of Polypeptide CH50 and Its Chemotaxis and Antitumor Function by Transfection in vivo

Objective: To investigate the correlation between the expression of vascular endothelial growth factor(VEGF) and angiogenesis in tumor. Methods: VEGF 165 sense and antisense gene recombinants were introduced into human gastric cancer cells, respectively. Then the growth of transfected cells in nude mice and the microvascular density and histological change were examined. Results: The growth rate of tumor in nude mice inoculated with sense VEGF cells was markedly higher than that in nude mice inoculated with antisense-VEGF cells. In histological examination, the microvascular density in tumor caused by sense-VEGF cells was greatly higher than that in tumor caused by antisense VEGF cells. Conclusion: As starting angiogenesis, VEGF might promote the growth of tumor, and the inhibition of VEGF production might prevent solid tumor from growing.