复方丹参滴丸对培养的乳鼠心肌细胞缺氧及缺氧/复氧时Fas/FasL蛋白表达的影响

探讨复方丹参滴丸 (DanShenPill,DSP)对培养的乳鼠心肌细胞缺氧及缺氧 /复氧时凋亡相关基因Fas/FasL蛋白表达的影响。方法 :按常规培养新生 4d乳鼠心肌细胞 ,于培养 2 4h后进行缺氧及缺氧 /复氧实验 ,以免疫组织化学方法检测心肌细胞Fas/FasL蛋白表达的变化。结果 :缺氧 4 5h及 10 5h后 ,心肌细胞Fas/FasL蛋白的阳性表达指数 (positiveexpressionindex ,PEI %)均显著高于对照。 10 5h组与 4 5h组无明显差异。复方丹参滴丸组PEI明显低于缺氧组 (P <0 0 5 )。缺氧 30min后再给氧 4h与 10h ,心肌细胞Fas/FasL蛋白的PEI显著高于对照 ,复氧 10h组与 4h组无明显差异 ,复方丹参滴丸组PEI低于缺氧复氧组 (P <0 0 5 )。结论 :缺氧及缺氧 /复氧时均有凋亡相关基因Fas及其配体FasL蛋白表达的增强 ,复方丹参滴丸可通过下调Fas/FasL蛋白表达以减少凋亡从而减轻缺氧损伤及缺氧 /复氧损伤。

Effect of Dan Shen Pill on expression of Fas/FasL proteins in cultured neonatal rat cardiomyocytes during hypoxia and reoxygenation

AIM: To observe the effect of Dan Shen Pill on expression of Fas/FasL proteins in cultured neonatal rat cardiomyocytes during hypoxia and reoxygenation. METHODS: The neonatal rat cardiomyocytes were isolated and cultured in normal condition for 24 hours before hypoxia and reoxygenation experiments were conducted. The expressions of Fas/FasL proteins in cultured neonatal rat cardiomyocytes were determined by immunohistochemistry and positive expression index (PEI) was calculated. RESULTS: (1) The PEI of Fas/FasL proteins in hypoxia group was higher, but the PEI in Dan Shen Pill treated group was significantly lower than that in control. (2) Under the condition of hypoxia for 30 min then reoxygenation for 4.5 h or 10.5 h, the PEI of Fas/FasL oprotein in cultured neonatal rat cardiomyocytes was also much higher than that in control. However, in the presence of Dan Shen Pill, PEI reduced remarkably compared to cells in the absence of Dan Shen Pill under the same hypoxia and reoxygenation conditions. CONCLUSION: The results indicated that the enhancement of Fas/FasL protein expression emerged in the hypoxia and reoxygenation injury in cultured neonatal rat cardiomyocytes. Dan Shen Pill treatment reduced apoptosis during hypoxia and reoxygenation by decreasing the Fas/FasL protein expression.