| 佛手GRAS基因的克隆及表达分析 |
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| 引用本文： | 石瑞,曹诣斌,陈文荣,郭卫东. 佛手GRAS基因的克隆及表达分析[J]. 浙江师范大学学报(自然科学版), 2011, 0(4): 446-451 |
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| 作者姓名： | 石瑞 曹诣斌 陈文荣 郭卫东 |
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| 作者单位： | 浙江师范大学化学与生命科学学院; |
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| 基金项目： | 浙江省自然科学基金资助项目(Y307472); 浙江省科技计划项目(2008C22002) |
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| 摘 要： | 利用抑制消减杂交法从芸香科柑橘属不耐寒植物佛手中分离得到了一个表达序列标签(EST)片段,结合RACE技术克隆获得该基因的1 669 bp序列,编码区长1 236 bp,编码413个氨基酸.通过Blastn同源序列比对分析,结果显示该基因与拟南芥已知的GRAS基因同源性较高;与GenBank数据库比对分析,表明该基因具有GRAS特有的保守结构域.因此,命名该基因为:CmsGRAS(GenBank登录号:JF440647).用荧光定量聚合酶链式反应(qPCR)的方法研究了该基因在低温胁迫处理下的表达特性,结果显示该基因在低温胁迫后的表达量有明显的变化.
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| 关 键 词： | 佛手 GRAS基因 克隆 低温 表达分析 |
On cDNA cloning and expression analysis of GRAS gene in fingered citron |
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| SHI Rui,CAO Yibin,CHEN Wenrong,GUO Weidong. On cDNA cloning and expression analysis of GRAS gene in fingered citron[J]. Journal of Zhejiang Normal University Natural Sciences, 2011, 0(4): 446-451 |
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| Authors: | SHI Rui CAO Yibin CHEN Wenrong GUO Weidong |
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| Affiliation: | SHI Rui,CAO Yibin,CHEN Wenrong,GUO Weidong(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China) |
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| Abstract: | A fragment of EST was isolated from fingered citron by suppression subtractive hybridization.It was cloned the full-length cDNA of the EST by RACE.The result showed that this fragment was highly associated with GRAS genes of Arabidopsis and the sequence alignment showed that it had a conserved domain of GRAS.Therefore,the GRAS gene of fingered citron could be named as CmsGRAS(GenBank accession number: JF440647),which had an ORF of 1 236 bp and encoded 413 amino acids.Fluorescence quantitative PCR method was... |
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| Keywords: | fingered citron GRAS gene clone cold stress expression analysis |
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