黑色素瘤缺乏因子2在小鼠急性胰腺炎模型中的表达

目的: 初步探讨黑色素瘤缺乏因子2（absent in melanoma 2, AIM2）在急性胰腺炎（acute pancreatitis, AP）模型中的表达。方法: 利用雨蛙素建立小鼠急性胰腺炎动物模型，实验分组为对照组与AP组，利用实时荧光定量PCR（Q-PCR）与Western blot检测两组动物外周血单核细胞（peripheral blood mononuclear cells,PBMCs）中AIM2，白介素-1β（IL-1β）与白介素-18（IL-18）mRNA基因与蛋白的表达情况；利用ELISA法检测两组动物外周血单核细胞培养基中IL-1β与IL-18的含量。结果: Q-PCR与Western blot结果显示，与对照组相比较，AIM2、IL-1β及IL-18 mRNA与蛋白在AP组PBMCs中的表达显著升高（P<0.01）；ELISA检测结果显示，AP组PBMCs合成分泌的IL-1β、IL-18的量显著高于对照组PBMCs合成分泌的量（P<0.01）。结论: AIM2在AP模型PBMCs的表达升高，表明其形成的炎症小体在这些细胞被激活，进而促进炎症因子IL-1β与IL-18的产生，因此，AIM2可能成为临床AP治疗潜在的治疗靶点。

Expression of absent in melanoma 2 in acute pancreatitis of mice model

AIM: To explore the expression of absent in melanoma 2 (AIM2) in pancreas in acute pancreatitis (AP). METHODS: Caerulein was used to set AP mice model, which were then divided into the control group and the AP group. Q-PCR and Western blot were used to detect AIM2, IL-1beta and IL-18 mRNA and protein expression in peripheral blood mononuclear cells (PBMCs). ELISA method was used to detect the concentration of IL-1beta and IL-18 in culture supernatants of PBMCs. RESULTS: Q-PCR and Western blot showed that compared with the control group, the expression of AIM2, IL-1β and IL-18 mRNA and protein in PBMCs AP group increased significantly (P<0.01). ELISA test results showed that compared with the control group, the concentration of IL-1beta and IL-18 from culture supernatants of PBMCs in AP group increased significantly (P<0.01). CONCLUSION: The higher expression of AIM2 in PBMCs of AP showed activation of the AIM2 inflammasome in PBMCs results in exacerbated IL-1beta and IL-18 production. Therefore, AIM2 might be a potential therapeutic target in clinical AP treatment.