CUL4A促进人胃癌细胞增殖、侵袭和转移的机制

目的:探讨CUL4A基因在胃癌增殖、侵袭和转移中的机制。方法:siRNA干扰胃癌SCG-7901细胞中CUL4A基因的表达；CCK-8法检测各组细胞的增殖情况；Transwell小室观察各组细胞的侵袭能力；Annexin-V-FITC/PI法检测各组细胞的凋亡情况，分析CUL4A对细胞凋亡的影响；Real time-PCR分析上皮间质转化（EMT）相关基因的表达情况。结果:siRNA有效干扰了胃癌SCG-7901细胞中CUL4A基因的表达（P＜0.05）；干扰CUL4A基因有效抑制了胃癌细胞的增殖（P＜0.05）；Transwell小室结果显示，干扰CUL4A基因阻碍了SCG-7901细胞的侵袭能力（P＜0.05）；细胞凋亡结果显示，干扰CUL4A基因促进了细胞的凋亡（P＜0.05）；Real time-PCR结果显示，干扰CUL4A基因后Snail mRNA和ZEB1 mRNA的表达降低（P＜0.05），E-cadherin mRNA的表达升高（P＜0.05）。结论:CUL4A基因促进胃癌细胞的增殖，抑制凋亡和侵袭，且影响了EMT相关基因的表达。

CUL4A promote human gastric cancer cell proliferation,invasion and metastasis and its mechanisms

Objective:To investigate the mechanism of CUL4A gene on the proliferation and invasion in gastric cancer.Methods:siRNA interference the expression of CUL4A in gastric cancer SCG-7901 cells.Using CCK-8 method to detect the proliferation of each group cells.To detect the invasion of each group cells by Transwell chambers.To detect each group cells apoptosis by Annexin V-FITC/PI method,and analysis the effects of CUL4A on cell apoptosis.Using Real time-PCR to analyze the expression of EMT related genes in gastric cancer cells.Results:siRNA interferenced the expression of CUL4A gene effectively in SCG-7901 gastric cancer cells（P＜0.05）.siRNA CUL4A inhibited the proliferation of gastric cancer cell（P＜0.05）.The results of Transwell chambers,interference CUL4A hindered the invasion of SCG-7901 cells（P＜0.05）.According to the results of cell apoptosis,siRNA CUL4A gene promoted the apoptosis of cells（P＜0.05）.Real time-PCR results showed that the expression of Snail mRNA and ZEB1 mRNA were lower than other group cells（P＜0.05）,the expression of E-cadherin mRNA was increased（P＜0.05）.Conclusion:CUL4A gene promoted gastric cancer cell proliferation,inhibited apoptosis and inhibition,and affected the expression of EMT related genes.