黄芪皂甙Ⅳ联合骨髓间充质干细胞移植对大鼠脑缺血/再灌注损伤海马神经元凋亡及相关基因表达的影响

目的 探讨黄芪皂甙Ⅳ联合骨髓间充质干细胞(BMSCs)移植对缺血再灌注大鼠海马神经细胞凋亡及Bcl-2、Bax、Caspase-3表达的影响.方法 实验动物随机分为假手术组、模型组、BMSCs组、BMSCs+黄芪组.采用线栓法建立大鼠大脑中动脉缺血(MCAO)模型,BMSCs组、BMSCs+黄芪组分别于建模成功后3 h...

Effects of Astragaloside IV combined with bone mesenchymal stem cell transplantation on neuron apoptosis and the expression of apoptosis related genes in hippocampus of cerebral ischemia-reperfusion rats

Objective To investigate the effects of Astragaloside IV combined with bone mesenchymal stem cells（BMSCs）on the apoptosis of neural cells and expressions of Bcl-2,Bax and caspase-3 in hippocampus of cerebral ischemia-reperfusion rats.Methods 42 healthy SD rats were randomly divided into sham operation,model,BMSCs and BMSCs＋ Astragaloside Ⅳgroups.The rat model were established by middle cerebral artery occlusion（MCAO）,PBS and PKH-26 labeled BMSCs suspension were injected via caudal vein 3 hours after MCAO.Astragaloside IV was administered 5min before cerebral ischemia,and 12h,24h after cerebral ischemia in BMSCs＋ Astragaloside Ⅳgroup.72h after reperfusion,neurological function score were observed,the expressions of Bcl-2,Bax and caspase-3 were determined by immunohistochemistry and real-time fluorescent quantitative PCR.The apoptosis of neural cells was detected by TUNEL method.Results PKH-26 labeled BMSCs were observed in hippocampus,the neurological scores in BMSCs＋ Astragaloside Ⅳgroup significantly decreased compared with model and BMSCs group,（P0.05）.The number of apoptotic neural cells and the expression of Bcl-2,Bax and caspase-3 proteins were significantly increased in model than in sham（P0.01）.Compared with model group,the number of apoptotic neural cells and the expression of Bax and caspase-3 proteins were significantly lower in BMSCs group and BMSCs＋ Astragaloside Ⅳgroup （P0.05）,but the expression of bcl-2 proteins was significantly increased （P0.05）.Moreover,the effect of BMSCs＋ Astragaloside Ⅳ is more powerful than BMSCs（P0.05）.The relative expression of Caspase-3,Bax mRNA was significantly lower in BMSCs＋ Astragaloside Ⅳgroup than in model and BMSCs groups （P0.05）,but the relative expression of Bcl-2 mRNA significantly increased by real-time fluorescent quantitative PCR （P0.05）.Conclusion BMSCs＋ Astragaloside Ⅳ could play coordinate inhibition on neural cell apoptosis in cerebral ischemia-reperfusion rats,which may be related to Bcl-2 and Caspase-3 and Bax expressions.