| 绿色荧光蛋白标记成人骨髓间充质干细胞的超微结构特征 |
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| 引用本文： | 马丽花,赵熠,蔡学敏,邓永丽,潘兴华.绿色荧光蛋白标记成人骨髓间充质干细胞的超微结构特征[J].中国组织工程研究与临床康复,2011,15(6). |
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| 作者姓名： | 马丽花 赵熠 蔡学敏 邓永丽 潘兴华 |
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| 作者单位： | 解放军昆明总医院干细胞与组织器官工程研究中心,云南省昆明市,650032 |
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| 基金项目： | 云南省自然科学基金资助项目(2003C00132)~~ |
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| 摘 要： | 背景:研究表明,绿色荧光蛋白标记的骨髓间充质干细胞免疫学表型、细胞周期、分化潜能等均未发生明显改变。目的:进一步观察绿色荧光蛋白标记对骨髓间充质干细胞超微结构的影响。方法:分离培养成人骨髓间充质干细胞,抗CD34、抗CD105免疫细胞化学染色鉴定,选第3代细胞进行绿色荧光蛋白标记,超薄切片后用透射电镜观察细胞超微结构,以未经绿色荧光蛋白标记骨髓间充质干细胞为对照。结果与结论:骨髓间充质干细胞表达CD105,不表达CD34。绿色荧光蛋白标记后,骨髓间充质干细胞细胞质发出绿色荧光,绿色荧光主要集中于细胞核周围的胞浆内,远离细胞核的胞浆内荧光强度逐渐减弱。相对于未标记的骨髓间充质细胞,经绿色荧光蛋白标记后细胞粗面内质网、高尔基体等细胞器较多,而线粒体相对较少;另外,脂滴和空泡样结构也多一些。结果证实绿色荧光蛋白对骨髓间充质干细胞性状的影响较为局限,是一种较为理想的细胞标记示踪剂。
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| 关 键 词： | 绿色荧光蛋白 骨髓间充质干细胞 超微结构 干细胞 标记 |
Ultrastructural characteristics of adult bone marrow mesenchymal stem cells labeled with green fluorescent protein |
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| Ma Li-hua,Zhao Yi,Cai Xue-min,Deng Yong-li,Pan Xing-hua.Ultrastructural characteristics of adult bone marrow mesenchymal stem cells labeled with green fluorescent protein[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(6). |
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| Authors: | Ma Li-hua Zhao Yi Cai Xue-min Deng Yong-li Pan Xing-hua |
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| Affiliation: | Ma Li-hua,Zhao Yi,Cai Xue-min,Deng Yong-li,Pan Xing-hua Research Center of Stem Cell,Tissue and Organ Engineering,Kunming General Hospital of Chinese PLA,Kunming 650032,Yunnan Province,China |
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| Abstract: | BACKGROUND:Studies have suggested that no significant changes were determined in immunological phenotype,cell cycle and differentiation potential of bone marrow mesenchymal stem cells(BMSCs) labeled with green fluorescent protein(GFP).OBJECTIVE:To further study effects of GFP on ultrastructure of BMSCs.METHODS:BMSCs were isolated from adult marrow and cultured in vitro.BMSCs were stained in immunocytochemistry with antibody of anti-CD34 and anti-CD105 to identify.The third generation of BMSCs were labeled w... |
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