18F-氟代脱氧葡萄糖诱导MCF-7细胞株的凋亡研究

目的研究18F-氟代脱氧葡萄糖(18F-FDG)诱导体外培养MCF-7乳腺癌细胞的凋亡情况。方法不同剂量18F-FDG加入体外培养MCF-7乳腺癌细胞中共培养,以γ计数仪测定细胞摄取18F-FDG的量;用流式细胞仪测定细胞早期和晚期凋亡率;用TUNEL法测定细胞凋亡的数量。结果①相同数量级的MCF-7细胞对18F-FDG的摄取量与剂量增加呈线性;②流式细胞结果示:经12 h18F-FDG孵育引起MCF-7的平均早期凋亡率为12.5%,平均晚期凋亡率为39.3%,总平均凋亡率为51.8%。经24 h18F-FDG孵育引起MCF-7的平均早期凋亡率为10.4%,平均晚期凋亡率为42.1%,总平均凋亡率为52.5%;③TUNEL显示:用凋亡指数(apoptotic index,AI)表示,与空白对照组AI(5.13±1.51)%相比,18F-FDG孵育12 h爬片的AI为(45.93±1.51)%,18F-FDG孵育24 h爬片的AI为(46.93±1.52)%。结论一定剂量范围内,18F-FDG对MCF-7细胞诱导凋亡效果显著。

Effect of 2-deoxy-2-[~(18)F] fluoro-D-glucose on apoptosis of human MCF-7 cells

Objective To investigate effect of 2-deoxy-2-^18F]fluoro-D-glucose on apoptosis of human MCF-7 cells.Methods Different doses of ^18F-FDG were co-cultured with MCF-7 in vitro.γ-ray high energy counting machine was used to count ^18F-FDG uptaking in MCF-7 cells.The cell apoptosis percent was detected by flow cytometry.The number of apoptosis cells was counted by using a TUNEL kit.Results ①In vitro,uptaking ^18F-FDG of the same number MCF-7 cells related with dose in line.②FCM displayed that after ^18F-FDG incubating for 12h the early average apoptosis percent was 12.5%,the later average apoptosis percent was 39.3%,and total average apoptosis percent was 51.8%.After ^18F-FDG incubating 24h,the early average apoptosis percent was 10.4%,the later average apoptosis percent was 42.1%,total average apoptosis percent was 52.5%.③TUNEL kit analysis showed that AI was（45.93±1.51）% after 18F-FDG incubating 12h,AI was（46.93±1.52）% after ^18F-FDG incubating 24h,compared with control of AI was（5.13±1.51）%.Conclusion Apoptosis of human MCF-7 cells was induced by moderate ^18F-FDG dose.