高效液相色谱法同时测定健康人口服加味逍遥散后血清阿魏酸和芍药苷含量及其药动学研究

目的：研究高效液相色谱法（high performance liquid chromatography,HPLC）同时测定健康人口服加味逍遥散（Modified Xiao-Yao Decoction,MXYD）后血清中阿魏酸和芍药苷含量及其药动学。方法：水浴法预处理血样,HPLC法同时测定8名健康人在口服加味逍遥散浓缩汤剂后8个时间点各个血清样本中芍药苷与阿魏酸的血药浓度。应用3P97药动学分析软件对血药浓度-时间数据进行拟合。选用香豆精为内标,芍药苷和阿魏酸检测波长分别为：0-10min为230nm和10-25min为320nm。结果：8名健康志愿者口服MXYD浓缩汤剂后,在血清中检测到芍药苷和阿魏酸。两种化合物分离完全,未出现其他物质干扰峰,保留时间（retention ti me,Tr）分别为8.02min和13.32min,内标香豆素为19.14min。芍药苷和阿魏酸的平均回收率分别为100.9%和95.3%,平均相对标准偏差（relative standarddeviation,RSD）分别为3.4%和3.2%。用空白血清加标准品绘制标准曲线,芍药苷线性范围为40-1280ng/ml,信噪比为3时最低检测线为5ng/ml,最低检测浓度为15ng/ml（RSD=12.5%）;阿魏酸线性范围为10-320ng/ml,最低检测线为0.65ng/ml,最低检测浓度5ng/ml（RSD=9.7%）。成分日间与日内精密度及准确度在92.1%-109.9%,且RSD〈5%。结论：采用高效液相色谱法对人体服用加味逍遥散后血清中的芍药苷和阿魏酸进行同步测定,适用于临床试验中血清芍药苷和阿魏酸的定性定量研究。

Simultaneous determination and pharmacokinetic studies of ferulic acid and paeoniflorin in human serum by high performance liquid chromatography after oral administration of Modified Xiao-yao Decoction

Objective:To simultaneously determine the contents and explore the pharmacokinetics of ferulic acid and paeoniflorin by high performance liquid chromatography(HPLC)after oral administration of Modified Xiao-yao Decoction(MXYD),a compound of traditional Chinese herbal medicine.Methods:A total of 8 healthy men were enlisted in this study.The serum samples were preprocessed by immersion method.The HPLC system was used to determine the contents of ferulic acid and paeoniflorin in the blood samples of the 8 heal...