| 利用离子液体[BMIM]2[CR]萃取牛血清蛋白的研究 |
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| 引用本文： | 娄本浊.利用离子液体[BMIM]2[CR]萃取牛血清蛋白的研究[J].陕西化工,2012(8):1323-1326. |
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| 作者姓名： | 娄本浊 |
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| 作者单位： | 陕西理工学院物理与电信工程学院,陕西汉中723003 |
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| 基金项目： | 国家自然科学基金（4110510）;陕西省自然科学基础研究计划基金（2012JM6016）;陕西省教育厅专项科研计划项目资助课题（11JK0553） |
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| 摘 要： | 利用离子交换过程将亲和性染料刚果红合成为BMIM]2CR]，并进行牛血清蛋白萃取实验。研究结果表明，BMIM]2CR]离子液体的浓度为10mmol／L，萃取时间为30min以及溶液呈酸性时，对牛血清蛋白的萃取效果最佳，可达90％以上。该研究可为BMIM]2CR]实际蛋白质萃取应用中提供一定的可行性条件。
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| 关 键 词： | 室温离子液体 刚果红 牛血清蛋白 萃取效率 |
Study on the BSA extraction by ionic liquid of [BMIM] 2 [CR] |
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| LOU Ben-zhuo.Study on the BSA extraction by ionic liquid of [BMIM] 2 [CR][J].Applied Chemical Industry,2012(8):1323-1326. |
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| Authors: | LOU Ben-zhuo |
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| Affiliation: | LOU Ben-zhuo ( School of Physics and Telecommunication Engineering, Shaanxi University of Technology, Hanzhong 723003, China) |
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| Abstract: | An environmental friendly ionic liquid BMIM] 2 CR] with affinity is synthesized by ionic exchange and the bovine serum albumin （BSA） is extracted by this new type of extracting agent. The results showed that the optimum 30 min of extraction time studied providing certain conditions of BSA extraction are 10 mmol/L of ionic and in acidic solution, and the extraction efficiency can feasibility conditions for protein extraction application. liquid concentration, be above 90%. This studied providing certain feasibility conditions for protein extraction application. |
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| Keywords: | room temperature ionic liquids Congo red bovine serum albumin extraction efficiency |
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