大鼠再狭窄模型的建立和内膜增生时相变化的观察

目的:为进一步探讨血管成形术后再狭窄的发生机制和血管损伤后内膜增生的时相变化。方法:取30只Sprague—Dawley大鼠行右颈总动脉球囊内膜剥脱术建立再狭窄动物模型，于第1，3，9和28，60天取实验动脉段和对侧正常动脉段行光镜、电镜观察及计算机图像分析。结果:实验发现在术后第3天内膜即明显增厚，并在第28天达到高峰，此后不再继续增加，同时通过电镜观察发现这种时相特征与平滑肌细胞的表型转化有关。结论:在大鼠再狭窄模型上的内膜增生与再狭窄发生过程类似，具有明显的时相特征，并与平滑肌细胞的表型转化有关。

The Observation on Time-dependent Characteristic and Inherent Mechanism of Intimal Thickening After Rat Carotid Arterial Injury

Objective To investigate the characteristic and the mechanism of intimal thickening at different times after rat carotid arterial injury. Methods Thirty Anesthetized rats underwent right carotid arterial endothelial injury by balloon catheter introduced via the external carotid arterial. Rats were divided into five subgroups, which were killed at 1, 3, 9, 28, 60 days after balloon denudation, respectively.Carotid arteries were observed under micros and electron microscope and were analyzed by computerized morphometry. Results Irregular thickening of arterial intima and stenosis of arterial cavity were observed since 3 days after balloon denudation. At the 28th day the intimal thickening reached a peak. The proliferation characteristic at different times was related to the phenotype transformation of smooth muscle cells.Conclusion It indicates that intimal thickening after rat carotid arterial injury have a distinct time-dependent characteristic which is related to the phenotype transformation of smooth muscle cells.