As2O3 诱导K562细胞凋亡过程中细胞表面电荷变化的研究

目的:研究三氧化二砷(As₂O₃) 诱导K₅₆₂ 细胞凋亡时细胞表面电荷的变化, 阐明As₂O₃ 诱导K₅₆₂细胞凋亡的可能机制, 为As₂O₃ 在临床上的应用提供理论依据。方法:应用细胞电泳仪检测As₂O₃ 诱导K₅₆₂细胞凋亡中细胞电泳率的变化。通过细胞增殖、活力检测, 形态学观察, 亚G1 期细胞含量和DNA 凝胶电泳等鉴定细胞凋亡。结果:1.0 ～ 20 μmol·L^-1As₂O₃ 作用于K₅₆₂细胞, 在细胞形态、DNA 凝胶电泳、FCM 显示出典型的细胞凋亡特征之前, 试验组细胞表面电荷已在1.6 h 左右开始下降, 6 h 内降低最明显, 6 h 后虽有下降, 但幅度明显减弱。与对照组比较, 低于1.0 μmol·L^-1 的As₂O₃ 对细胞表面电荷影响不大。结论:细胞表面电荷的下降是K₅₆₂ 细胞凋亡的早期事件。细胞表面电荷的下降有一定的时间范围, 超过此范围, 细胞表面电荷下降趋向无限大。

Changes of cell surface charge of K562 cells during apoptosis induced by arsenic trioxide

AIM: To explore the changes of cell surface charge on apoptotic K₅₆₂ induced by arsenic trioxide (As₂O₃).METHODS: The changes of cell surface charge were observed by the electrophoresis mobility.The techniques used to estimate apoptotic activity in K₅₆₂ cells involved cell proliferation, cell viability, cell morphology, flow cytometry, and agarose gel electrophoresis assay. RESULTS: The electrophoresis mobility decreased with the passing of time.The decrease appeared at about 1.6 h and greatly enhanced in 6 h, which occurred before the typical changes of cell morphology and DNA contents for apoptosis.After 6 hours, the reduce in electrophoresis mobility was not obvious, in spite of decrease.CONCLUSION: The changes of cell surface charge can reflect apoptosis, especially in early time.It is early event of apoptotic K₅₆₂.