白花前胡提取物对腹主动脉缩窄大鼠心室重构及Bcl-2、Bax 蛋白表达的影响

目的:研究白花前胡提取物(PdE) 对腹主动脉缩窄术后大鼠心室重构及心肌组织凋亡相关蛋白Bcl-2、Bax 表达的影响。方法:部分缩窄大鼠腹主动脉术制作心肌肥厚模型。称重法检测左室心肌重量, 计算心系数;观察细胞组织结构改变, 测量左心室内径;免疫组织化学法检测凋亡相关基因及Bcl-2、Bax 蛋白表达。结果:腹主动脉缩窄术后, 大鼠左室重量及心系数明显增加;400 %PdE 可明显减轻大鼠腹主动脉术所致左室重量增加(用药组0.49 ±0.07 g, 模型组0.57 ±0.16 g, P <0.05) 及心系数的升高(用药组2.44 %, 模型组2.82 %, P <0.05);200 % PdE 亦可明显降低大鼠腹主动脉术后心系数的升高(用药组2.51 %, 模型组2.82 %, P <0.05);腹主动脉缩窄术后大鼠心肌细胞明显肥大,胞核呈浓染、增大, 细胞间距增大, 组织横断面心肌纤维增多;400 % PdE 及200 % PdE 可有效改善因腹主动脉缩窄所致心肌组织结构变化;腹主动脉缩窄术后, 大鼠左室腔内径明显缩小, 而400 % PdE 及200 % PdE 可显著改善大鼠腹主动脉术后左室腔内径缩小(P <0.05);模型组Bcl-2 与Bax 蛋白表达率与伪手术组、各浓度PdE 组比较均有显著性差异(P<0.05), 但伪手术组, 400 %及200 % PdE 组Bcl-2/Bax 表达比例均较模型组有明显增加(P <0.05) 。结论:PdE 能有效改善因腹主动脉缩窄所致的心室重构过程中的左心室壁肥厚和心系数增加, 调节心肌细胞凋亡相关蛋白Bcl-2 与Bax 的表达。

Effects of Peucedanum praeruptorum Dunn Extract on left ventricular remodeling and Bcl-2, Bax protein expression in aorta coarctation rats

AIM: To investigate the effects of Peucedanum praeruptorum Dunn Extract on left ventricular remodeling and apoptosis-related protein(Bcl-2, Bax) expression in aorta coarctation rats.METHODS: Partly aorta coarctation was practiced in Wistar rats.The weight of the left ventricular and the cardiac index were measured;the diameter of the left ventricular cavity was measured by means of HE dyeing;the expression of Bcl-2 and Bax protein in myocardium was observed by immunohistochemical method.RESULTS: After partly aorta coarctation, the weight of the left ventricular and the cardiac index of rats were increased significantly.Compared with the model group, 400 % PdE decreased the left ventricular and the cardiac index effectively and 200 % PdE decreased the increasing cardiac index in rats which after aorta coarctation.In the model group, the myocardial cells were much bigger and there were much more parallel connections and series connections of sarcomere;compared with those in the model group, the myocardial cells were nearly normal or just had a slight degree of hypertrophy and hyperplasia in both 400 % PdE and 200 % PdE groups.Compared with the model group, the 400 % PdE and 200 %PdE groups had bigger diameter of left ventricular cavity, the difference was significant (P <0.05); compared with the model group, 400 % and 200 % PdE significantly increased the proportion of Bcl-2 and Bax protein expression in the myocardial cells of rats after the aorta coarctation.CONCLUSION: Peucedanum praeruptorum Dunn Extract can affect the remodeling process of ventricular muscles and the apoptosis-related proteins in different ways and had positive importance in treatment of ventricular remodeling.Therefore, it deserves further researching and developing.