茄子品种遗传多样性的RAPD检测与聚类分析

用RAPD分子标记的方法对来自不同国家的34份茄子品种进行遗传多样性分析,从120条RAPD引物中筛选出有效的22条引物分别对34份茄子品种进行扩增,共检测出232个等位基因位点,每条引物平均检测出10.5个,其中192个为多态位点,多态位点比率为82.76%。POPGENE结果分析表明,Nei's基因多样性H为0.2756,Shannon指数为0.4145,显示出丰富的遗传多样性。计算得出的Jaccard相似系数变化范围为0.331～0.805,根据Jaccard相似系数和组内连接法建立的系统聚类图,34份茄子大致可分为两大类型:圆茄类型和长茄类型,这与经典的形态学分类基本上相符,从而从分子水平上支持了以果形作为茄子品种分类指标的观点。

Clustering Analysis and Identification of Genetic Diversities in Eggplant (Solanum melongena L.) Varieties with RAPD

Thirty-four eggplant (Solanum melongena) varieties from different countries were studied for the analysis of genetic diversity by RAPD.Twenty-two effective primers were screened from 120 RAPD arbitrary primers,and the total of 232 alleles were detected,in which there were 192 alleles polymorphic (82.76% polymorphim),the average number of alleles per primer was 10.5.The result of POPGENE analysis indicated that Nei's gene diversity H was 0.2756,Shannon's information index was 0.4145,and the highly diverse was displayed obviously.The calculated Jaccard's similarity values ranged from 0.331 to 0.805.According to the date,the dendrogram using within-group linkage method showed that the 34 varieties of eggplants were grouped into two clusters:round-shape and long-shape.The result was consistent with morphological taxon,which supported the taxonomy of eggplant based on fruit-shape on molecular level.