干扰素 γ加强肿瘤坏死因子相关凋亡诱导配体抑制神经母细胞瘤细胞的作用

目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人神经母细胞瘤的作用及干扰素 γ(IFN γ)对新型凋亡分子TRAIL抗瘤活性的影响，并探讨其影响机制。方法应用MTT分析和流式细胞仪研究TRAIL对人神经母细胞瘤细胞的抑制作用及IFN γ对TRAIL抗瘤活性的影响。用RT PCR方法检测IFN γ作用48h后，Caspase 8 mRNA的表达。结果TRAIL(10,50,100μg/L)单独作用、IFN γ(1000U/mL)单独作用后，对SY5Y细胞的增殖抑制率分别为(438±089)%、(354±166)%、(503±126)%、(533±206)%；凋亡率分別为(518±333)%、(526±364)%、(500±288)%、(814±735)%。IFN γ(1000U/mL)与TRAIL(100μg/L)联合作用后，对SY5Y细胞的增殖抑制率为(4122±709)%；凋亡率为(2129±620)%。RT PCR方法发现IFN γ作用48h后Caspase 8 mRNA的表达明显上调。结论神经母细胞瘤细胞SH SY5Y对TRAIL不敏感，IFN γ可以明显提高TRAIL对神经母细胞瘤细胞的敏感性，其发生机制可能是通过IFN γ上调Caspase 8 mRNA的表达而实现的。

The research progress of the etiology in preterm premature rupture of membrane

AbstractObjectiveTo explore the effect of TRAIL on neuroblastoma cell line and the influence of IFN γ on the antitumor effects of TRAIL.MethodsUse MTT and flow cytometric analysis to detect the antitumor effects of TRAIL and study the effects of IFN γ on TRAIL in inhibiting human neuroblastoma cells.ResultsThe rates of killing of TRAIL alone、IFN γ alone and the combination of TRAIL and IFN γ were (503±126)%、(533±206)%、(4122±709)%,IFN γ could enhance the killing rate of TRAIL to the SY5Y cells.FCM analysis suggested that IFN γ enhanced the killing rate of TRAIL to the SY5Y cells based on inhibiting apoptosis of SY5Y cells.ConclusionSH SY5Y cells are not sensitive to TRAIL.IFN γ can upregulate the apoptosis of SY5Y induced by TRAIL.