Ｘ线照射诱导人胃癌ＳＧＣ-７９０１细胞生物特性改变以及ＹＫＬ ４０基因表达变化

目的　观察Ｘ线照射后胃癌ＳＧＣ-７９０１细胞形态改变、细胞周期分布情况以及是否可以诱导ＹＫＬ-４０基因的表达。方法　将胃癌细胞株ＳＧＣ-７９０１分为空白对照组（不照射）和实验组（２Ｇｙ／１ｆ和４Ｇｙ／２ｆ），实验组细胞经６ ＭＶＸ线照射后取两组细胞在倒置显微镜下观察细胞形态学的改变，流式细胞术检测ＳＧＣ-７９０１细胞周期的变化情况，半定量ＲＴ-ＰＣＲ检测基因ＹＫＬ-４０ｍＲＮＡ的表达。结果　实验组（２Ｇｙ／１ｆ）细胞经Ｘ线照射后出现明显的形态学改变；流式细胞检测发现，与空白对照组细胞比较，２Ｇｙ、４Ｇｙ实验组细胞Ｓ期上调，Ｇ₂／Ｍ期下调，组间比较差异有统计学意义（Ｐ＜０.０５）。ＲＴ-ＰＣＲ检测胃癌细胞株ＳＧＣ-７９０１不表达ＹＫＬ-４０或检测不到ＹＫＬ-４０ｍＲＮＡ，经Ｘ线照射后ＹＫＬ-４０表达。结论　Ｘ线照射可使人胃癌细胞发生形态改变及细胞增殖周期再分布，并且可诱导人胃癌细胞表达ＹＫＬ-４０。

Changes of biological characteristics and expression of YKL-40 gene in gastric cancer SGC-7901 cell induced by X-ray irradiation

Objective To observe the morphological changes,the division of cells cycle and expression of YKL-40 gene level in gastric cancer SGC-7901 cells after irradiation by X-ray.Methods SGC-7901 cells were devided into control group（no irradiation）and experimental group（2Gy/1fraction and 4Gy/2fraction）.SGC-7901 cells in the two groups were taken.Morphological changes of the tumor cells by invert microscop,divisions of cell cycle by FCM and changes of YKL-40 mRNA of SGC-7901 cells by RT-PCR assay in experimental grop were observed compared to control group.Results Morphology of SGC-7901 cells in 2Gy/1 fraction group showed significant changes after irradiation.Cell S phase up and G2/M phase down in 2Gy/1 fraction group and 4Gy/2 fraction group,there was significant difference between the two groups（P〈0.05）.Meanwhile,expression of YKL-40 mRNA could be induced by irridiation.Conclusion We find that human gastric cancer SGC-7901 cells changed in morphology and redistributed in cell cycle after irradiation of X-ray.Inaddition,it can increase the expression of YKL-40.