p53基因敲除小鼠生长发育和繁殖性能的调查

目的 观察p53基因敲除小鼠与BALB/c小鼠在生长发育和繁殖性能方面的差异.方法 p53基因敲除小鼠和BALB/c小鼠各34只,雌雄各半,观察其2周龄至12周龄的体质量和体长,并随机选取30笼一雌一雄所产第2胎仔鼠进行幼仔个数的统计.结果 在生长发育方面,3周龄时p53基因敲除小鼠与BALB/c小鼠体质量无明显差异,P=0.846＞0.05；6周龄时p53基因敲除小鼠体质量明显低于BALB/c小鼠,P=0.000＜ 0.05；体长在3周龄和6周龄时p53基因敲除小鼠体长明显低于BALB/c小鼠,P=0.000 ＜0.05.在繁殖性能方面,p53基因敲除小鼠同样明显低于BALB/c小鼠,P=0.000＜0.05.结论 初步研究了p53基因敲除对小鼠的繁殖与发育的影响,与BALB/c小鼠比较均有显著差异.

A Survey of Growing Development and Reproductive Performance of p53 Knockout Mice

Objective To investigate the differences in growing development and reproductive performance between p53 knockout mice and BALB/c mice.Method 34 p53 knockout mice with half males and half females and 34 BALB/c mice （half male and female） were adopted in the trial,and their body weight and body length were measured from 2 to 12 weeks of age.Litter sizes at the second parity from 30 pairs of mice were checked.Result The difference in body weight between 3-week old p53 knockout mice and BALB/c mice was not significant （P ＞ 0.05）,and body weight of 6-week old p53 knockout mice was significantly lower than that of 6-week old BALB/c mice （P ＜0.05）.Body length of 3-week and 6-week old p53 knockout mice was significantly lower than that of 3-week and 6-week old BALB/c mice （P ＜ 0.05）.Reproductive performance of p53 knockout mice was significantly lower than that of BALB/c mice （P ＜ 0.05）.Conclusion p53 gene knockout could exert significant effect on the growing development and reproductive performance of the mice.